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EXPERIMENTAL RATIONALE FOR THE USE OF FLUIDS WITH DIFFERENT REDOX POTENTIAL AS A BASIS FOR INFUSION THERAPY

Introduction: The hypothesis is that a change in the redox potential (Red-Ox) of infusion solutions can increase their pharmacological efficacy. Objectives: To study the parameters of redox potential (ORP or Red-Ox) and pH of infusion solutions. To identify the general biological properties of ionized liquids with different ORP when administered by different methods to experimental animals and when applied to the wound surface. To study the effects of infusion therapy with solutions based on ionized fluids with various ORP in anaphylactic shock, bacterial sepsis, alcoholic hepatitis, dehydration, and skin injuries. Materials and methods: Determination of practical osmolarity of ionized liquids with different ORP was carried out by the cryoscopic method with the use of the Beckmann thermometer. The study used experimental models of anaphylactic shock, bacterial sepsis, alcoholic hepatitis, dehydration and cutaneous injuries in rats. The study of the general biological properties of ionized liquids with different ORP was carried out in 4 groups of experiments. In the first group: experiments to investigate the effect of liquids with different ORP on the action of ophthalmic drugs and eye tissue – on 75 rabbits; in the 2-nd: experiments on the level of glucose in the blood; in the 3-rd: the study of reactions of the cardiovascular system to intravenous administration of ionized liquids with various ORP in the maximum permissible volumes; in the 4-th: the research of regenerative abilities of liquids with various ORP with a cutaneous injury. Results: It was found that the ORP of infusion solutions is higher than the potential of liquid media by 100-400 mV. The cryoscopic method established the possibility of preparing isoosmolar infusion solutions with various Red-Ox potentials. In the models of anaphylactic shock and bacterial sepsis, the expediency of changing the Red-Ox potential of body fluids is shown. Addition of ionized liquids with different ORP to a 0.1% solution of adrenaline hydrochloride in a ratio of 1:1 leads to prolongation of the pharmacological effects of the drug. After instilling the ionized liquid with positive ORP in the conjunctiva a weak local anesthetic effect is observed. Electrocardiography of the experimental rabbits showed that a decrease in the Red-Ox potential of the isotonic sodium chloride solution increases its therapeutic range, reducing the load on the right atrium and ventricles. Regenerative efficiency in the treatment of wound surfaces was noted when using ionized liquid with ORP = minus 250 mV. Conclusion: The use of ionized fluids with various ORP as the basis of infusion solutions for anaphylactic shock, bacterial sepsis, alcoholic hepatitis, and dehydration has shown high efficiency. At the same time, the issues of compatibility of ionized liquids with different ORP with various pharmacological preparations remain unresolved, the issues of pharmaceutical stability of liquids with negative Red-Ox potential, etc. are not solved either, which indicates the prospects of research in this direction.

Иллюстрации

Fig. 1. The impact of liquids with different ORP for a change of the length of the wound on the 7th day.

Note * - differences statistically significant compared to the control group (p<0.05), n=7.

 

Table 1.  The influence of the ionized fluid with various ORP on the dynamics of changes in body weight and urine output of rats with rehydration therapy (M±m, n=10).

 

Fig. 2. The influence of ionized liquids on the morphological picture of liver of rats. The control group (a), a group of ionized liquid with a negative redox potential (b) and ionized fluid with a positive redox potential (c) on the 7th day after the acute alcohol poisoning. Colouring with hematoxylin-eosin, x100.

Table 2. The effect of the liquid on the Distribution of animals in each study group according to severity of anaphylaxis and mortality (%).

 

Table 3. The impact of fluids of various ORP on the percentage of bacteria in the blood (n=20)

Fig. 3. The impact of liquids with different ORP on the condition of the tissues of the kidneys of rabbits upon the intravenous administration. а - control group (0.9% sodium chloride solution), b - liquid, ionized liquid with a negative redox potential and c - ionized fluid with a positive redox potential in terms of staphylococcal sepsis. Colouring with hematoxylin-eosin, х100.

Fig. 4. The impact of liquids with different ORP on the condition of the liver tissue of rabbits being injected intravenously. а - control group (0.9% sodium chloride solution), b - liquid, ionized liquid with a negative redox potential and c - ionized fluid with a positive redox potential in terms of staphylococcal sepsis. Colouring with hematoxylin-eosin, х100.

DOI: 10.18413/2313-8971-2017-3-2-29-37
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