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PHARMACOLOGICAL EFFECTS OF THE SYNTHETIC ANALOGUE OF ANTIMICROBIAL PEPTIDE OF INDOLICIDIN 21 ON THE REGENERATION OF THERMAL WOUNDS IN THE EXPERIMENT

Introduction: Burn injuries in developed countries are among the most spread injuries of peacetime. The growth of resistance of microorganisms to existing antibiotics makes it necessary to develop some new generation antimicrobial drugs in the coming years. One possible way to solve the problem is to create drugs based on antibiotic peptides. Methods: There were studied the effects of the use of the synthetic analogue of indolicidin 21 on the processes of regeneration of thermal wounds, the systemic inflammatory response, the functional activity of neutrophils and macrophages, lipid peroxidation processes for IIIA-B degree burns and II-III degree frostbites in rats. Results: In the course of the study, we have established some stimulating effects of the synthetic analogue of the natural antimicrobial peptide indolicidin 21 on the processes of reparative skin regeneration after a local skin burn IIIA-B and a local frostbite of II-III degree, which are manifested in the acceleration of healing and prevention of purulent complications of thermal wounds. The use of the analogue of the indolicidin 21 has positive effects both on the functional activity of granulocytes of blood and macrophages, and on the systemic inflammatory reaction. The use of the synthetic analogue leads to an increase in the concentration of lipid peroxidation products in the blood plasma and skin supernatant throughout the experiment after a local burn, and up to 10 days after a local frostbite. The activity of antioxidant enzymes under the influence of the synthetic analogue of indolicidin was higher than the control values. Conclusion: The study has revealed that the use of synthetic analogue of indolicidin 21 is an effective method of pharmacological correction of reparative processes in the skin with thermal damage. It was also found that the most effective use of the synthetic analogue of the peptide was with the dose of 500 μg/kg.

Иллюстрации

Fig. 1. The preparation of the burn wound site on the 14th day after the burn injury in the control group. Mallory, X 100. There is a "crawling" of the newly formed epithelium under the scab. The loose connective tissue is weakly expressed. The fibers of the mesh layer of the dermis and hypodermis are edematous.

Fig. 2. The preparation of the burn wound site for the 14th day after the burn injury in the experimental group, which received a synthetic analogue of indolicidin at a dosage of 500 µg/kg. Mallory, X 100. There is no fibrous necrotic scab. The surface of the wound is covered with a pronounced layer of the newly formed epithelium. The underlying loose connective tissue is highly pronounced. The fibers of the mesh layer are structural.

Fig. 3. The preparation of the cold wound site on the 14th day after the burn injury in the control group. Mallory, X 400. A fibrous necrotic scab formed. Epithelization is "crawling". The underlying loose connective tissue is slightly expressed. The fibers of the mesh layer of the dermis are edematous.

 Fig. 4. The preparation of the cold wound site on the 14th day after the burn injury in the experimental group, which received a synthetic analogue of indolicidin at a dosage of 500 μg/kg. Mallory, X 400. There is no scab, the formed layer of the newly formed epithelium covers the fibrin films. The underlying loose connective tissue is highly pronounced. The fibers of the mesh layer are structural.

Table 1. The content of lipid peroxidation products in blood plasma of control and experimental groups after burn injury

Table 2. The effect of a synthetic analogue of indolicidin on the concentration of lipid peroxidation products in the skin and adjacent tissues in control and experimental animals after local frostbite

DOI: 10.18413/2313-8971-2017-3-2-38-49
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