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STUDY OF PHARMACOLOGICAL ACTIVITY OF NIGELLA DAMASCENA FIXED OIL IN EXPERIMENT

Abstract

Introduction: Literature data, rich chemical macro-microelement composition set the stage for experimental pharmacological study of adaptogene and lipid protective activity of Nigella damascena essential oil.

Objectives: study of pharmacological activity of Nigella damascena essential oil while simulating pathology on laboratory animals.

Methods: irritant action – HET-CAM test and DRAIZE-test, acute toxicity, open field test, simulated hypoxia method, swimming with the load, No-way-out and Rotating-rod tests, acute lipid pathology under Tween 80 (tween lipid pathology), vitamin D2 hyperlipidemia, simulated chronic heart failure (CHF).

Results and discussion: Nigella damascena essential oil have low irritant effect along with almost non-toxicity. Nigella oil increase life duration with the presence of hypoxia and endurance while swimming-with-the-load test. Course administration of Nigella oil normalize hormone-mediatore metabolism on the background of acute stress. Course administration of Nigella damascena essential oil normalize lipids on the background of CHF, acute tween and subchronic vitamin D2 hyperlipidemias.

Conclusion: stress protective, actoprotective and adaptogene properties of Nigella damascena essential oil was pharmacologically proved. It was substantiated experimentally that Nigella damascena essential oil has an impact on lipid metabolism on the background of chronic heart failure (CHF), acute tween, subchronic vitamin D2 lipid pathologies and blood serum lipoprotein lipase (LPL) activity.

Иллюстрации

Fig. 1. The influence of Nigella damascena essential oil on nervous system.

Note: NDEO – Nigella damascena essential oil, * - p>0,05 reliable relatively to untreated animals.

 

Fig. 2. Ergotropic effect escalation after Nigella damascena essential oil administration at the doses from 1 to 25 mg/kg.

Note: NDEO – Nigella damascena essential oil, min – minutes.

Fig. 3. The influence of Nigella damascena essential oil 10 mg/kg on muscular load and hypoxia.

Note: NDEO – Nigella damascena essential oil; min. – minutes; sec. – seconds; * - p>0.05 reliable relatively to control animals; # - p>0.05 reliable relatively to animals who got NDEO 10mg/kg one-time.

Fig. 4. Stress protective activity of Nigella damascena essential oil at a dose of 10 mg/kg and 1 mg/kg.

Note: NDEO – Nigella damascena essential oil; cour – course administration; GM – gastric mucosa; # - p>0.05 reliable relatively to control animals.

Fig. 5. The influence of Nigella damascena essential oil on hormone-mediator metabolism under simulated acute stress.

Note: U – untreated; O – one-time administration; C – course administration; AD – adrenaline; NAD – noradrenaline; DA – dopamine; H – histamine; S – serotonin; * - p>0.5 reliable relatively untreated animals

Table 1.  The influence of Nigella damascena essential oil 10 mg/kg on 11-OXY metabolism in rat’s blood under acute stress

Fig. 6. The influence of Nigella damascena essential oil on rat's lipid metabolism under chronic heart failure.

Note: NDEO – Nigella damascena essential oil; Ch – cholesterol; HDLP – high-density lipoprotein; LDLP – low-density lipoprotein; VLDLP – very low-density lipoprotein; 1-time – one-time administration; Cour. – course administration; * - p>0.05 reliable relatively untreated animals; # - p>0.05 – reliable relatively control animals

Fig. 7. Lipid metabolism values changing in blood serum and liver after Nigella damascena essential oil administration under acute tween lipid pathology.

Note: NDEO – Nigella damascena essential oil; 1-time – one time administration; cour. – course administration; path. – pathology; BS – blood serum; L – liver; * - p>0.05 reliable relatively untreated animals; # - p>0.05 reliable relatively control animals.

Fig. 8. Lipid metabolism values changing in blood serum (BS) and liver (L) after Nigella damascena essential oil administration at a dose of 10 mg/kg under subchronic vitamin D2 lipid pathology.

Note: NDEO – Nigella damascena essential oil; 1time – one-time administration; cour. – course administration; path. – pathology; BS – blood serum; L – liver; * - p>0.001 difference reliability relatively untreated animals; # - p>0.001 difference reliability relatively control animals

Fig. 9. The influence of Nigella damascena essential oil at a dose of 10 mg/kg on blood serum lipoprotein lipase activity.

Note: NDEO – Nigella damascena essential oil; 1time – one-time administration; cour. – course administration; path. – pathology; * - p>0.05 reliable relatively untreated animals; # - p>0.05 reliable relatively control animals.

DOI: 10.18413/2313-8971-2017-3-4-89-99
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