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CORRECTION OF RETINAL ANGIOPATHY OF HYPERTENSIVE TYPE BY DIMETHYLAMINOETHANOL DERIVATIVE 19-16 IN EXPERIMENT

Introduction: 63% of hypertensive patients have evidence of hypertensive angiopathy. Increase of effectiveness of drug therapy of retinal angiopathy of hypertensive type is an important task of pharmacology.

Objectives: To increase the effectiveness of pharmacological correction of retinal angiopathy of hypertensive type using a new dimethylaminoethanol (DMAE) derivative 19-16.

Materials and methods: We studied protective effects of DMAE derivative 19-16 in doses 25 mg/kg, 50 mg/kg on a model of retinal angiopathy of hypertensive type on Wistar male rats, which was carried out by N-nitro-L-arginine-methyl ester (L-NAME) introducing in a dose 12.5 mg/kg/day within 28 days. To study the fundus of experimental animals, direct ophthalmoscopy was used. Electroretinography (ERG) was recorded in response to a single stimulation. The induced biopotentials were amplified, averaged and presented graphically with the help of Biopac-systems MP-150 with the program AcqKnowledge 4.2 (USA). To assess the degree of development of functional damage to the retina, the ratio of the amplitudes of the b- and a-waves, the coefficient b/a, was estimated.

Results and discussion: DMAE derivative 19-16 in a dose 50 mg/kg prevented the development of ischemic damage and retinal vascular changes caused by introduction of L-NAME, to a greater extent than in a dose 25 mg/kg. The observed retinoprotective effects are confirmed by ophthalmoscopy and ERG.

Conclusion: The search of new ways of correction of the retinal angiopathy as a complication of hypertension is an urgent task of pharmacology, which can be solved by using a new DMAE derivative 19-16.

Иллюстрации

Fig. 1. Example of ophthalmoscopy on intact Wistar rat. Optic disc is circular or oval shape and stands out from the fundus in pale - pink. The boundaries of the optic nerve disc are clear. It lies in the plane of the retina. From the middle of the optic nerve exit the central vessels of the retina. Blood vessels of the retina don`t have anastomoses. The veins and arteries are straightforward, caliber is uniform, not crimped. The general background is pink.

Fig. 2. Example of ophthalmoscopy on Wistar rat with retinal angiopathy of hypertensive type (control). Optic disc is circular or oval shape and stands out from the fundus in pink. The boundaries of the optic nerve disc are clear. Veins are congested, full-blooded, crimped at the periphery. Arteries are narrowed, slightly crimped. Retina is palely (ischemic). Symptom Salus-Hun I.

Fig. 3. Example of ophthalmoscopy on animal with correction by DMAE derivative 19-16 in a dose 50 mg/kg/day. Optic disc is circular or oval shape and stands out from the fundus in pale - pink. The boundaries of the disc are clear. Blood vessels of the retina don`t have anastomoses.  The veins and arteries are straightforward, no crimping. Veins are slightly dilated. The general background is pink, not ischemic ter> a dose 50 mg/kg/day is shown in fig. 3.

Table 1. Results of electroretinography on day 29th of the experiment (M ± m; n = 10), r.u.

DOI: 10.18413/2313-8971-2017-3-4-113-119
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