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CORRECTION OF ISCHEMIC DAMAGE TO THE RETINA ON APPLICATION OF PHARMACOLOGICAL PRECONDITIONING OF RECOMBINANT ERYTHROPOIETIN

To study the protective properties of pharmacological preconditioning with recombinant erythropoietin suberitro stimulating dose of 50 IU / kg in the retina of the eye, was the model of retinal ischemia, ischemia with the assessment of the temporary simulation mode of the retina of rats with the instrumental methods of analysis and morphometric studies. The most suitable model was a model 30 minute ischemia with subsequent reperfusion periods of 1 hour to 72 hours. We studied the protective effect preconditioning suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, preconditioning distant ischemic and emoxipine 2 mg/kg per rat model of retinal ischemia. In the experiment, it was revealed that the recombinant erythropoietin (50 IU/kg) prevents the development of degenerative retinal layers due to ischemic damage more than distant ischemic preconditioning and emoxipine. The observed protective effect of erythropoietin us with the development of ischemia was confirmed by laser Doppler flowmetry, electroretinography and morphometry. Using suberitro stimulating recombinant erythropoietin in a dose of 50 IU/kg, 30 minutes prior to the pathology simulation eliminates erythropoietic effect, and the lack of positive dynamics in groups with preliminary administration of glibenclamide 5 mg/kg, the key role of ATP-dependent potassium channels in a preconditioning mechanism implementation. Identification and use of pharmacological agents, which have the effect of preconditioning, may be a new approach in the correction and prevention of retinal ischemia, which is the leading element in the pathogenesis of a number of visual pathologies. The possibility of pharmacological preconditioning with erythropoietin ischemic lesions of the retina is essential for the development of anti-ischemic agents for the treatment and prevention of ocular pathologies of ischemic origin.

Иллюстрации

Figure 1. Level retinal microcirculation 1 hour after retinal ischemia simulations.

Note. * - p <0.05 compared with the group of intact animals

Figure 2. The level of the microcirculation in the retina after 72 h after modeling retinal ischemia

Note. * - p <0.05 compared with the group of intact animals.

Figure 3. Evaluation of retinal electrophysiological state 1 hour after modeling retinal ischemia.

Note. * - p <0.05 compared with the group of intact animals.

 

Figure 4. Assessment of retinal electrophysiological state 72 hours after modeling retinal ischemia.

Note. * - p <0.05 compared with the group of intact animals.

 

Figure 5. An example of measuring the thickness of the inner nuclear layer of the retina intact animal. H & E stain. Increasing the X400.

Figure 6. Example of measurement of the thickness of the retinal photoreceptor layer is intact animal eyes. H & E stain. Increasing the X400.

Figure 7. An example of morphometric measurements of the inner nuclear layer of the retina thickness rats 1 hour after reperfusion disease modeling (30 minute model). Ochre. hematoxylin and eosin. Increase 0 x40. 

Figure 8. Example morphometric measurement of the thickness of the retinal photoreceptor layer of the rat at 1 hour after reperfusion disease modeling (30 minute model). H & E stain. Increasing the X400

Table 1. The thickness of the inner nuclear layer and retinal photoreceptors in rat experimental groups after 1 h reperfusion (M ± m; n = 10)

Figure 9. Example morphometric measurement of the thickness of the inner nuclear layer of the retina of rat after 72 hours of reperfusion with a moment disease modeling (30 minute model). H & E stain. Increasing the X400.

Figure 10. Example morphometric measurements of the retinal photoreceptor layer thickness rats 72 hours after reperfusion disease modeling (30 minute model). H & E stain. Increasing the X400. 

Table 2. The thickness of the inner nuclear layer and retinal photoreceptors in rat experimental groups, after 72 h of reperfusion (M ± m; n = 10)

 

Table 3. The level of the microcirculation in the retina of rats After 1 h of reperfusion (M ± m), PE

Table 4. The level of the microcirculation in the retina after 72 h of reperfusion (M ± m), p.u.

Table 5. The results of evaluation of retinal electrophysiological state after 1 h of reperfusion (M ± m; n = 10)  

Table 6. The results of evaluation of retinal electrophysiological state after 72 h of reperfusion (M ± m; n = 10)

Figure 11A. Electroretinogram retina of rats: A - ERG retina intact animal.

Figure 11B. Electroretinogram retina of rats: B - ERG animal retinal ischemia (inhibition observed b-wave of electroretinogram) after 72 h of reperfusion.

 Figure 12A. Retina ERG in rats after 72 h of reperfusion in the experimental groups: A - correction of the EPO.

Figure 12B. Retina ERG in rats after 72 h of reperfusion in the experimental groups: B - correction DIP.

 

Figure 13A. ERG retina of rats after 72 h of reperfusion in the experimental groups: A - control + glibenclamide.

 Figure 13B. ERG retina of rats after 72 h of reperfusion in the experimental groups: B - correction DIP + glibenclamide.

 Figure 14A. ERG retina of rats after 72 h of reperfusion in the experimental groups: A - EPO correction + glibenclamide.

 Figure 14B. ERG retina of rats after 72 h of reperfusion in the experimental groups: B - correction emoxipin.

Figure 15. Example morphometric measurement of thickness of the inner nuclear layer of the retina rats 1 hour after reperfusion of EPO on a background correction. H & E stain. Increased X400.

 

Figure 16. Example of morphometric measurements of the retinal photoreceptor layer thickness rat through 1:00 reperfusion on the background correction of EPO. H & E stain. Increasing the X400.

Table 7. Morphometric indices retinal layers experimental animals 1 hour after reperfusion (M ± m; n = 10)

Table 8. Morphometric indices retinal layers experimental animals at 72 hours of reperfusion (M ± m; n = 10)

DOI: 10.18413/2313-8971-2016-2-2-67-90
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