THE INFLUENCE OF ELECTROLYTES OF THE IONS AND THE INHIBITOR ON THE ACTIVITY OF TOTAL ATPASE OF ERYTHROCYTES OF BROILER CHICKENS
The total ATPase activity of erythrocytes of broiler chickens depends on the concentration of ions Na+ and K+. The ATPase activity at 76.5% due to magnesium ions, 96.5% of the sodium ions and 47.6% of potassium ions. Maximum ATPase activity was observed in incubation medium containing ions: Na+ 120 mmol/ml, K+ 20 mmol/ml; Mg2+ - 3.0 mmol/ml and was of 9,240,23 nmol •mg protein-1•min-1. The ATPase activity has no effect of the specific inhibitor – strophanthin-K in the concentration range of 0-100 mg•l-1 in the medium containing ions Na+ and K+, and in an environment without them.
Figure 1. The effect of sodium ions on the activity of ATPase, (n=10), P≤0,05
Figure2. The influence of potassium ions on the ATPase activity, (n=10), P≤0,05
Figure 3. Influence of magnesium ions on the activity of ATPase, (n=10), P≤0,05
Table 1. The effect of combinations of ion composition on the activity of ATPase, (n=10)
Figure 4. The effect of strophanthin-K on the activity of ATPase in the absence of Na+ ions and K+ in the medium containing these ions in optimum concentration (n=10), P≤0,05
Figure 5. Regression analysis of the effect of strophanthin-K on ATPase activity in the absence of Na+ ions and K+.
Figure 6. Regression analysis of the effect of strophanthin-K on ATPase activity in the medium containing ions Na+ and K+.
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Mosyagina, I.P. The influence of electrolytes of the ions and the inhibitor on the activity of total atpase of erythrocytes of broiler chickens / I.P. Mosyagina, V.V. Mosyagin // Research result: pharmacology and clinical pharmacology. – 2016. – Vol.2, №2. – P. 113-118