Animals

Test-objects

Study design

Biomaterial preparation

Acute toxicity determination

Cerebral ischemia model

Morris water maze test

Assessment of changes in the rate of cerebral blood flow

Necrosis zone evaluation

Assessment of the activity of mitochondrial complexes

Measurement of citrate synthase activity

Determination of lactic acid concentration

Determination of pyruvic acid concentration

Determination of ATP concentration

Statistical analysis

Assessment of acute toxicity of the test extract

Evaluation of the effect of the test extract on changes in the behavior of animals in the Morris water maze test

Evaluation of the effect of the test extract on the change in the cerebral blood flow rate, the size of the brain necrosis zone, and the concentrations of lactic and pyruvic acids in the blood serum of rats

Evaluation of the effect of the test extract on the change in the activity of the complexes of the mitochondrial respiratory chain, citrate synthase, and the concentration of ATP in the brain of rats