Chemicals

Experimental animals

Isolated perfused kidney (IPK) procedure

Acute blood pressure studies in normotensive rats

Estimation of nitric oxide in kidney perfusate

Estimation of fumarate in kidney perfusate

Statistical analyses

Fumarate reduced perfusion pressure in the pre-constricted isolated kidney

Inhibition of fumarase evoked an increase in perfusion pressure

Inhibition of fumarase increased fumarate levels and nitric oxide production in the isolated kidneys

Acute bolus doses of fumarate reduced blood pressure and cortical blood flow (CBF) but increased medullary blood flow (MBF) in normotensive rats

Acute bolus injection of a fumarase inhibitor, PMA, altered renal and systemic hemodynamics in normotensive rats

Effect of the inhibition of fumarase on fumarate-induced changes on MABP, CBF and MBF

Effect of N(ω)-nitro-L-arginine methyl ester (L-NAME) on fumarate-induced changes in MABP, CBF and MBF