The effect of derivatives of tetrahydropyrido[2,1-b][1,3,5] thiadiazine on hematologic indices of rats with subacute parotitis

Abstract

Introduction: Regardless of belonging of NSAIDs to one or another chemical group, they all have common side effects that can occur when using these drugs for a long or short period. One type of toxicity in the spectrum of side effects of modern non-steroidal anti-inflammatory drugs is hematotoxicity.

Objectives: to study the indices characterizing the red and white hematopoiesis in a clinical blood examination in animals with a simulated inflammation process against a background of pharmacocorrection with original thiadiazine derivatives.

Methods: The experiments were carried out on 48 white pedigree mature rats of both sexes weighing 170–210 g. The tetrahydropyrido [2,1-b] [1,3,5] thiadiazine derivatives II, III and V were selected, since they showed the strongest anti-inflammatory, analgesic and antipyretic properties in the previous experiments. The animals were divided into eight groups: intact (rats without the pathology), control (with inlammation), referent 1 (inflammation+diclofenac sodium 0.5 mg/kg), referent 2 (inflammation+analgin 5 mg/kg), referent 3 (inflammation+indomethacin 5 mg / kg) and three test groups (with test substances administered at a dose of 5 mg/kg). Administration of the drugs was carried out for 14 days at the above doses. The standard methods were used to determine the number of erythrocytes, hemoglobin, color index, ESR, leukocytes and neutrophils.

Results: In the analysis of the numerical results of the experiment, the valid ranges of values were determined for most parameters under study, which made it possible to use nonparametric statistical methods, including the Wilcoxon signed-rank test, to evaluate the reliability of differences. The use of tetrahydropyrido [2,1-b] [1,3,5] thiadiazine derivatives II, III, V in animals with experimental parotitis was accompanied by an increase in the number of erythrocytes in comparison with that in the control group.

Conclusion: The studies of three derivatives of tetrahydropyrido [2.1-b] [1.3.5] thiadiazine, which have a high anti-inflammatory activity, proved that the compounds III and V have no hematotoxicity.