Maximum tolerant dose and analgesic activity of PT1 peptide

Authors

DOI:

https://doi.org/10.3897/rrpharmacology.5.38520

Abstract

Introduction: The article presents the results of the study of the maximum tolerant dose (MTD) and the analgesic activity of peptide PT1 isolated from Alopecosa marikovskyi spider venom. PT1 is the first compound of polypeptide nature, capable of exerting a selective modulating effect on purinergic P2X3 receptors.

Materials and methods: The study was conducted on 174 ICR mice. The analgesic activity of the peptide was evaluated in a thermal hypersensitivity test triggered by CFA and in a model of chemical irritation.

Results and discussion: The determined MTD for the peptide PT1 when administered intravenously provides evidence to attribute it to low-toxic compounds. The maximum analgesic activity of PT1 using the biomodel of hypersensitivity induced by CFA when tested 15 minutes after the administration was recorded at doses of 0.1 and 0.5 mg/kg. In the visceral pain test, the maximum analgesic activity 15 minutes after the administration of the chemical stimulus was observed at a dose of 0.01 mg/kg.

Conclusions: According to the results of testing peptide PT1, it is shown that it belongs to low-toxic compounds, has a pronounced analgesic activity in a wide range of doses of 0.0001–10 mg/kg.

Keywords:

P2X3 receptors, analgesics, pain models

Author Contribution

Yuliya A. Palikova, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Researcher, Laboratory of Biological Tests.

Viktor A. Palikov, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Researcher, Laboratory of Biological Tests.

Igor A. Dyachenko, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

PhD of Biological Sciences, senior researcher, Laboratory of Biological Tests.

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Published

30-09-2019

How to Cite

Palikova YA, Palikov VA, Dyachenko IA (2019) Maximum tolerant dose and analgesic activity of PT1 peptide. Research Results in Pharmacology 5(3): 37–42. https://doi.org/10.3897/rrpharmacology.5.38520

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Section

Review article

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