Effects of Dimephosphone on skin survival in conditions of reduced blood circulation

Authors

  • Pavel A. Galenko-Yaroshevsky Kuban State Medical University ORCID logo https://orcid.org/0000-0003-0873-284X
  • Denis A. Nefedov S. Fyodorov Eye Microsurgery Federal State Institution
  • Anait V. Zelenskaya Kuban State Medical University
  • Ivan I. Pavlyuchenko Kuban State Medical University
  • Elena N. Chuyan V.I. Vernadsky Crimean Federal University
  • Marina Yu. Ravaeva V.I. Vernadsky Crimean Federal University
  • Nafishet K. Tkharkahova Kuban State Medical University

DOI:

https://doi.org/10.3897/rrpharmacology.4.31880

Abstract

Introduction: The search for and creation of drugs with dermatoprotective and metabotropic activity is one of the priorities of modern diabetology. Synthetic organophosphorus compounds with no anticholinesterase activity, to which Dimephosphone belongs to, deserve great attention in this respect.

Materials and Methods: Experiments included 355 white non-linear male mice (18-34 g) and 799 male rats (150–305 g). The dermatoprotective activity (DPA) of Dimephosphone regarding the survival of a skin graft was studied against the background of normoglycemia, as well as against the background of experimental diabetes complicated by hypercholesterolemia. The study of microhemodynamics in the skin was performed using laser Doppler flowmetry. The effects on metabolic processes and the antioxidant system were studied by determining the levels of glucose, urea, creatinine, total bilirubin, total cholesterol, triglycerides, total protein, albumin, globulin, catalase, malondialdehyde, superoxide dismutase, glutathione reductase, glutathione and glutathione peroxidase.

Results: Dimephosphone has a pronounced DPA in conditions of reduced blood circulation against the background of normoglycemia and experimental (alloxan) diabetes complicated by exogenous hypercholesterolemia. By DPA, in most cases against the background of normoglycemia Dimephosphone exceeds Actovegine, is comparable to or inferior to Trental and Mexidol, and is more significant in terms of the therapeutic width than all the drugs taken for comparison.

Discussion: According to the obtained data , DPA of Dimephosphone may be due to its ability to exhibit significant vasodilating, antihypoxic, antioxidant, antiaggregant, membrane-stabilizing, anti-acidotic, antimicrobial and other properties and also to exert a normalizing effect on carbohydrate, protein, lipid and energy metabolism

Conclusion: Dimephosphone can be recommended for further preclinical and clinical studies in the form of various dosage forms, as well as in a combination therapy for metabolic disorders.

Keywords:

dimephosphone, skin, reduced blood circulation, experimental (alloxan) diabetes, exogenous hypercholesterolemia

Author Contribution

Pavel A. Galenko-Yaroshevsky, Kuban State Medical University

Corresponding member of The Russian Academy of Medical Sciences, Doctor of Medical Sciences, Professor, Head of the Department of Pharmacology . The author provided consultations on the planning, design and
implementation of the experiment. 

Denis A. Nefedov, S. Fyodorov Eye Microsurgery Federal State Institution

Ophthalmologist. The author set goals and objectives of the study, analyzed literature data, was directly involved in carrying out all phases of experimental work and writing an article.

Anait V. Zelenskaya, Kuban State Medical University

PhD in Medicine, Associate Professor of the Department of Pharmacology. The author participated in the simulation of experimental diabetes mellitus and the study of the antihyperglycemic activity of Dimephosphone.

Ivan I. Pavlyuchenko, Kuban State Medical University

Doctor of Medical Sciences, Professor, Head of the Department of Biology with a course of medical genetics. The author participated in the design of the experiment and in statistical data processing.

Elena N. Chuyan, V.I. Vernadsky Crimean Federal University

Doctor of Biological Sciences, Professor, Head of the Department of Human and Animal Physiology and Biophysics. The author conducted biochemical analysis and interpreted the obtained data.

Marina Yu. Ravaeva, V.I. Vernadsky Crimean Federal University

PhD in Biology, Associate Professor, Department of the Human and Animal Physiology and Biophysics. The author participated in the experimental evaluation of the effect of dimephosphone on microcirculation and assessment of skin flap survival in experimental animals.

Nafishet K. Tkharkahova, Kuban State Medical University

6 th year student of the Pediatric Faculty. The author participated in the assessment of skin flap survival in experimental animals, simulation of experimental diabetes mellitus complicated by hypercholesterolemia and blood sampling in animals.

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Published

06-12-2018

How to Cite

Galenko-Yaroshevsky PA, Nefedov DA, Zelenskaya AV, Pavlyuchenko II, Chuyan EN, Ravaeva MY, Tkharkahova NK (2018) Effects of Dimephosphone on skin survival in conditions of reduced blood circulation. Research Results in Pharmacology 4(4): 41–52. https://doi.org/10.3897/rrpharmacology.4.31880

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Section

Review article

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