The effect of new forms for external application on the vasodilating function of the endothelium and the concentration of endothelial nitric oxide synthases in rats with an experimental model of a pathological scar at early healing times

Abstract

Introduction: According to statistical data, the tendency towards an increase in the number of patients with keloid and hypertrophic scars at the site of skin lesions remains in the world, which in most cases is due to the lack of effective methods for preventing this type of complications. Therefore, the development of new methods of pharmacological correction, which allow us to normalize the wound healing process at an early stage, seems very relevant. One of the promising areas for the search for substances suitable for the prevention of pathological scars is the study of natural compounds possessing endothelioprotective activity, since, according to modern ideas, it is the endothelial dysfunction that underlies the pathogenesis of this complication.

Materials and Methods: All studies were performed on 60 male rats of the Wistar line, divided into 6 experimental groups. New forms for external use based on flavonoids PMFI-92 and PMFI-93 and comparison preparations "Kontraktubeks", "Egallohit" were used as research objects. The effect of new PMFI-92 and PMFI-93 formulations on the vasodilating function of the endothelium of the skin of animals with chemical burn was introduced with the introduction of nitric oxide synthesis modifiers: acetylcholine (ACS), L-arginine, nitro-L-arginine methyl ester (L-NAME). The effect of local pharmacological support in this pathology on the level of endothelial (eNOS), neuronal (nNOS) and inducible (iNOS) nitric oxide syntheses was determined by the method of solid phase enzyme immunoassay (ELISA).

Results and Discussion: Application of the proposed new forms based on natural compounds PMFI-92 and PMFI-93 allows to improve the vasodilating function of the endothelium already at the early stages of wound healing, with an increase in the concentration of eNOS and nNOS, as well as a decrease in the concentration of iNOS.

Conclusion: The experimental data obtained by us testify to the pronounced positive effect of the local application of PMFI-93 and PMFI-92 compositions on the vasodilating function of the skin vessels, and also to restore the balance between the activities of various forms of nitric oxide synthases.