Correction of retinal ischemic injuries by using non-selective imidazoline receptor agonists in the experiment

Authors

  • Еlena A. Levkova Clinic of Ambulatory Surgery Plus LLC
  • Anton L. Pazhinsky Clinic of Ambulatory Surgery Plus LLC
  • Sergey S. Lugovskoy St. Joseph Belgorod Regional Clinical Hospital
  • Anna A. Peresypkina St. Joseph Belgorod Regional Clinical Hospital
  • Victoria V. Bashuk Belgorod City Hospital №2
  • Leonid V. Pazhinsky Clinic of Ambulatory Surgery Plus LLC
  • Mikhail A. Martynov Belgorod City Hospital №2
  • Evgeniya A. Beskhmelnitsyna St. Joseph Belgorod Regional Clinical Hospital

DOI:

https://doi.org/10.3897/rrpharmacology.5.38498

Abstract

Introduction: Retinoprotective effects of non-selective imidazoline receptor agonists: potassium salt of С7070; sodium salt of С7070; С7070 processed with CO2 – were investigated in comparison with C7070 on the retinal ischemia-reperfusion model in rats.

Materials and methods: The protective effects of the substances were evaluated by using ophthalmoscopy, laser Doppler flowmetry, electroretinography, histological and morphometric studies of retinal layers.

Results and discussion: The most pronounced retinoprotective effect was observed in potassium salt of C7070 at a dose of 10 mg/kg, which expresses in approaching the normal eye fundus image, achieving the target values of the retinal blood flow, b/a coefficient, and reaching the norm values of morphometric indicators. A less pronounced protective effect was found in sodium salt of C7070 at a dose of 10 mg/kg, which expresses in a 71% decrease (p < 0.05) in semi-quantitative assessment of the eye fundus changes, an increase in the retinal blood flow level by 70.4% (p < 0.05), in b/a by 94% (p < 0.05) in comparison with the group without correction, and reaching the norm of the morphometric indicators. A retinoprotective effect of the substance C7070 processed with CO2 at a dose of 10 mg/kg is inferior to that of the sodium salt of C7070.

Conclusion: The retinoprotective activity of the substances is expressed in descending order: potassium salt of С7070 (10 mg/kg) ≈ С7070 (50 mg/kg) > sodium salt of С7070 (10 mg/kg) > С7070 processed with CO2 (10 mg/kg) ≈ С7070 (10 mg/kg). Injections of glibenclamide leveled the neuroretinoprotective effects of the substances to varying degrees, which confirmed the participation of ATP-dependent potassium channels in the implementation of these effects.

Keywords:

retinal ischemia-reperfusion, midazoline receptor agonists, C7070, ATP-dependent potassium channels, Wistar rats

Author Contribution

Еlena A. Levkova, Clinic of Ambulatory Surgery Plus LLC

Ophthalmologist.

Anton L. Pazhinsky, Clinic of Ambulatory Surgery Plus LLC

Otorhinolaryngologist.

Sergey S. Lugovskoy, St. Joseph Belgorod Regional Clinical Hospital

Doctor for X-Ray Endovascular Diagnosis and Treatment of the Department of X-Ray
Surgical Methods of Diagnosis and Treatment.

Anna A. Peresypkina, St. Joseph Belgorod Regional Clinical Hospital

Candidate of Biological Sciences, PhD in Biology, Pharmaceutist.

Victoria V. Bashuk, Belgorod City Hospital №2

Candidate of Medical Sciences, PhD in Medicine, Head of Ophthalmology Department.

Leonid V. Pazhinsky, Clinic of Ambulatory Surgery Plus LLC

Doctor of Medical Sciences, Otorhinolaryngologist.

Mikhail A. Martynov, Belgorod City Hospital №2

Surgeon, Surgical Department №2.

Evgeniya A. Beskhmelnitsyna, St. Joseph Belgorod Regional Clinical Hospital

Gynecologist, Department of Birth №2.

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Published

30-12-2019

How to Cite

Levkova ЕA, Pazhinsky AL, Lugovskoy SS, Peresypkina AA, Bashuk VV, Pazhinsky LV, Martynov MA, Beskhmelnitsyna EA (2019) Correction of retinal ischemic injuries by using non-selective imidazoline receptor agonists in the experiment. Research Results in Pharmacology 5(4): 7–17. https://doi.org/10.3897/rrpharmacology.5.38498

Issue

Section

Review article

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