Pathomorphological analysis of the gum tissues in the application of the combination of Soderm®-Forte gel with Cytoflavin® in experimental periodontitis in rats

Authors

DOI:

https://doi.org/10.18413/rrpharmacology.9.10017

Abstract

Introduction: Periodontitis is one of the most urgent problems of modern dentistry. The development of new paradigms and regimens of combination therapy of patients with periodontitis is a strategic task for pharmacologists and dentists. In view of this, pathomorphological examination is of high importance, since it allow us to conclude about the therapeutic effectiveness of the administered drugs with high objectivity.

Aim of the study: to evaluate the effect of the composition of Soderm®-Forte and Cytoflavin® on the pathomorphological pattern of gum tissues of rats with experimental periodontitis (EP).

Materials and Methods: EP was simulated in rats by ligature method. Study design: animals with intact periodontium; animals with untreated EP; animals with EP treated with traditional drug therapy (TDT); animals with EP treated with the combination of TDT and Soderm®-Forte gel; and animals with EP treated with the combination of TDT, Soderm®-Forte and Cytoflavin®. For pathomorphological examination, biopsy specimen was taken from the gingival margin of the lower incisors. Slides were stained with hematoxylin and eosin, as well as by Masson. Computer morphometry was performed using the ImageJ software.

Results: In EP, TDT has a moderate positive effect on pathomorphological changes in the gum. The combination of TDT and Soderm®-Forte and, to a greater degree, the combination of TDT, Soderm®-Forte and Cytoflavin® have high therapeutic efficacy, characterized by rapid regeneration of the gum tissues.

 Conclusion: The combination of TDT, Soderm®-Forte and Cytoflavin® in EP has a more pronounced therapeutic effect, manifested by early regression of pathological changes and acceleration of tissue regeneration in the gum.

Graphical Abstract

Keywords:

Soderm®-Forte gel, computer morphometry, pathomorphological changes in gum tissue, Cytoflavin®, experimental periodontitis

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Author Contribution

Pavel A. Galenko-Yaroshevsky, Kuban State Medical University

Сorresponding member of the Russian Academy of Sciences, Holder of an Advanced Doctorate (Doctor of Science) in Medical Sciences, Professor, Head of the Department of Pharmacology of Kuban State Medical University, Krasnodar, Russia; Senior Research Scientist of Research Institute for the Pharmacology of Living Systems of Belgorod State National Research University, Belgorod, Russia; е-mail: Galenko.Yaroсhevsky@gmail.com; ORCID ID https://orсid.org/0000-0001-6856-1777. The author’s contribution: concept development – conceptualization and direction development of the research; generation of the key aims and objectives.

Aleksandr А. Slavinskiy, Kuban State Medical University

Holder of an Advanced Doctorate (Doctor of Science) in Biological Sciences, Professor, Head of the Department of Pathological Anatomy of Kuban State Medical University, Krasnodar, Russia; е-mail: al-slavinsky@mail.ru; ORCID ID https://orcid.org/0000-0001-9824-9186. The author’s contribution: defining the key aims and objectives; interpretation of the data obtained; critical revision of the manuscript and provision of valuable comments.

Mark A. Zadorozhniy, Rostov State Medical University

Postgraduate student of the Department of Dentistry No.1 of Rostov State Medical University, Rostov-on-Don, Russia; e-mail: markys-97@yandex.ru; ORCID ID https://orcid.org/0000-0002-7528-6109. The author’s contribution: experimentation – collection, analysis and interpretation of the data obtained; drafting the manuscript.

Valeriy K. Leontev, A.I. Yevdokimov Moscow State University of Medicine and Dentistry

Member of the Russian Academy of Sciences, Holder of an Advanced Doctorate (Doctor of Science) in Medical Sciences, Professor of A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia; Vice-President of the Dental Association of Russia, Honored Scientist of the Russian Federation, laureate of the State Prize of the Russian Federation in Science and Technology; e-mail: LeontyevVK@mail.ru; ORCID ID https://orcid.org/0000-0003-2296-8904. The author’s contribution: approval of the final stage of the article preparation – admission of responsibility for all stages of the research, the integrity of the article and its final draft.

Artur V. Kheigetyan, Rostov State Medical University

PhD in Medical Sciences, Associate Professor, Head of the Department of Dentistry No.1 of Rostov State Medical University, Rostov-on-Don, Russia; e-mail: artur5953@yandex.ru; ORCID ID https://orcid.org/0000-0002-8222-4854. The author’s contribution: concept development – conceptualization and direction development of the research; generation of the key aims and objectives.

Viktor L. Popkov, Kuban State Medical University

Holder of an Advanced Doctorate (Doctor of Science) in Medical Sciences, Professor, Department of Orthopedic Dentistry of Kuban State Medical University, Krasnodar, Russia; e-mail: popkoff2015@yandex.ru; ORCID ID https://orcid.org/0000-0001-7362-0073. The author’s contribution: development and design of methodology, defining the key aims and objectives, critical revision of the manuscript and provision of valuable comments.

Anait V. Zelenskaya, Kuban State Medical University

PhD in Medical Sciences, Associate Professor of the Department of Pharmacology of Kuban State Medical University, Krasnodar, Russia; е-mail: anait_06@mail.ru; ORCID ID https://orcid.org/0000-0001-9512-2526. The author’s contribution: analysis and interpretation of research results; preparing and editing the article text; participation in the scientific design.

Lyudmila M. Chuprinenko, Kuban State Medical University

PhD in Medical Sciences, Associate Professor of the Department of Pathological Anatomy of Kuban State Medical University, Krasnodar, Russia; e-mail: lmchbox@mail.ru; ORCID ID https://orcid.org/0000-0002-2770-3529. The author’s contribution: pathomorphological analysis, analysis and interpretation of research results

Aleksandr А. Verevkin, Kuban State Medical University

PhD in Medical Sciences, Associate Professor of the Department of Pathological Anatomy of Kuban State Medical University, Krasnodar, Russia; e-mail: vilehand@bk.ru; ORCID ID https://orcid.org/0000-0002-4159-2618. The author’s contribution: computer morphometry, analysis and interpretation of the research results.

Izabella G. Lomsadze, Kuban State Medical University

3rd year undergraduate student of the Department of General Medicine of Kuban State Medical University, Krasnodar, Russia; e-mail: izabella.lomsadze@yandex.ru; ORCID ID https://orcid.org/0009-0007-5584-3397. The author’s contribution: literature search, pathomorphological analysis.

Mariya Yu. Materenchuk, I.M. Sechenov First Moscow State Medical University

4rd year undergraduate student majoring in Medical Biochemistry at Institute of Biodesign and Complex System Modelling of I.M. Sechenov First Moscow State Medical University, Moscow, Russia; e-mail: mariamatter231@gmail.com; ORCID ID https://orcid.org/0000-0002-0711-4153. The author’s contribution: graphic design and visualization of study design.

Svetlana A. Lebedeva, I.M. Sechenov First Moscow State Medical University

Holder of an Advanced Doctorate (Doctor of Science) in Biological Sciences, Professor of the Department of Pharmacology of Institute of Pharmacy of I.M. Sechenov First Moscow State Medical University, Moscow, Russia; e-mail: Lebedeva502@yandex.ru; ORCID ID https://orcid.org/0000-0003-0325-6397. The author’s contribution: defining the key aims and objectives; critical revision of the manuscript and provision of valuable comments.

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Published

19-04-2023

How to Cite

Galenko-Yaroshevsky PA, Slavinskiy AА, Zadorozhniy MA, Leontev VK, Kheigetyan AV, Popkov VL, Zelenskaya AV, Chuprinenko LM, Verevkin AА, Lomsadze IG, Materenchuk MY, Lebedeva SA (2023) Pathomorphological analysis of the gum tissues in the application of the combination of Soderm®-Forte gel with Cytoflavin® in experimental periodontitis in rats. Research Results in Pharmacology 9(2): 1–10. https://doi.org/10.18413/rrpharmacology.9.10017

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Experimental Pharmacology

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