Protective effects of thymogen analogues, modified by D-alanine, in hydrazine liver damage

Authors

DOI:

https://doi.org/10.18413/rrpharmacology.10.458

Abstract

Introduction: It is known that the insertion of D-amino acids into the structure of a peptide molecule can increase its half-life. The pharmacological drug Thymogen was modified with D-alanine from the N- and C-terminus of the molecule. The aim of the investigation was to study the reparative and antioxidant activity of structural analogues of thymogen in toxic liver damage by hydrazine in rats.

Materials and Methods: The investigation was conducted on 40 Wistar rats. Acute toxic liver damage was simulated with a single intraperitoneal injection of hydrazine hydrochloride at a dose of 50 mg/kg. Thymogen and its modified D-Ala analogues were administered intraperitoneally in equimolar doses for 5 days after intoxication. The animals were removed from the experiment 12 hours after the final administration of the peptides.

Results and Discussion: It has been established the reparative and antioxidant activities of thymogen structural analogues increased in conditions of liver damage with hydrazine. Peptides with the insertion of D-Ala reduced the level of free radical reactions more significantly in comparison with thymogen. All peptides comparably increased catalase activity in blood plasma and liver homogenate.

Conclusion: Thymogen analogues modified with D-Ala from the N- and C-terminus of the molecule can be considered as promising pharmacological substances for the development of new hepatoprotective drugs.

Graphical Abstract

Keywords:

thymogen, D-alanine, toxic liver damage, hydrazine, reparative activity of hepatocytes, free radical oxidation, catalase

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Author Contribution

Anna A. Chulanova, Kursk State Medical University

Teaching Assistant of the Biochemistry Department, Kursk State Medical University, Kursk, Russia; e-mail: chulanofs@mail.ru; ORCID ID https://orcid.org/0000-0003-0990-0390. The author developed the study design, conducted the experiments, analyzed and interpreted the data, statistically processed the data, and wrote the manuscript.

Angelina M. Smakhtina, Kursk State Medical University

Postgraduate student of the Pharmacology Department, Kursk State Medical University, Kursk, Russia; e-mail: smaxtina2012@yandex.ru; ORCID ID https://orcid.org/0000-0001-8458-3925. The author conducted the experiments, analyzed and interpreted the data, statistically processed the data, and wrote the manuscript.

Galina S. Mal, Kursk State Medical University

Doctor of Medical Sciences, Professor, Head of the Pharmacology Department, Kursk State Medical University, Kursk, Russia; e-mail: mgalina.2013@mail.ru; ORCID IDhttps://orcid.org/0000-0003-1712-5005. The author developed the study design, analyzed and interpreted the data, reviewed the manuscript and provided the critical insight.

Igor I. Bobyntsev, Kursk State Medical University

Doctor of Medical Sciences, Professor, Head of the Pathophysiology Department, Kursk State Medical University, Kursk, Russia; e-mail: bobig@mail.ru; ORCID ID https://orcid.org/0000-0001-7745-2599. The author developed the study design, analyzed and interpreted the data, reviewed the manuscript and provided the critical insight.

Ekaterina S. Mishina, Kursk State Medical University

PhD in Medicine, Associate Professor of the Histology, Cytology and Embryology Department, Kursk State Medical University, Kursk, Russia; e-mail: mishinaes@kursksmu.net; ORCID IDhttps://orcid.org/0000-0003-3835-0594. The author conducted histological studies, analyzed and interpreted the data.

Mikhail Yu. Smakhtin, Kursk State Medical University

Doctor of Biological Sciences, Professor of the Biochemistry Department, Kursk State Medical University, Kursk, Russia; e-mail: m.yu.smakhtin@mail.ru; ORCID ID https://orcid.org/0000-0001-7372-1819. The author developed the study design, analyzed and interpreted the data reviewed the manuscript and provided the critical insight.

Pavel M. Kopeykin, Limited Liability Company "IQ Chemical"

PhD in Biology, General Director, Limited Liability Company ”IQ Chemical”, Saint Petersburg, Russia; e-mail: pmkopeikin@gmail.com; ORCID ID https://orcid.org/0000-0002-6076-8842. The author chemically synthesized the analogues of thymogen.

Elena G. Bogomolova, Limited Liability Company “IQ Chemical”

PhD in Biology, Head of the laboratory, Limited Liability Company “IQ Chemical”, Saint Petersburg, Russia; e-mail: elenabogomolova000@gmail.com; ORCID IDhttps://orcid.org/0000-0003-4047-4086. The author chemically synthesized the analogues of thymogen.

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Published

07-06-2024

How to Cite

Chulanova AA, Smakhtina AM, Mal GS, Bobyntsev II, Mishina ES, Smakhtin MY, Kopeykin PM, Bogomolova EG (2024) Protective effects of thymogen analogues, modified by D-alanine, in hydrazine liver damage. Research Results in Pharmacology 10(2): 11–16. https://doi.org/10.18413/rrpharmacology.10.458

Issue

Vol. 10 No. 2 (2024)

Section

Experimental Pharmacology

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Copyright (c) 2024 Anna A. Chulanova, Angelina M. Smakhtina, Galina S. Mal, Igor I. Bobyntsev, Ekaterina S. Mishina, Mikhail Yu. Smakhtin, Pavel M. Kopeykin, Elena G. Bogomolova

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