First discovered effect of L-norvaline on tissue saturation and the activity of respiratory chain enzymes in ischemic and reperfusion injury of the small intestine

Authors

DOI:

https://doi.org/10.18413/rrpharmacology.11.861

Abstract

Introduction: Prevention and treatment of ischemic and reperfusion injury plays an important role in correcting the pathological manifestations of acute mesenteric thrombosis. The development of effective therapy and the study of the mechanisms of pharmacological agents’ action are an important problem faced by researchers.

Materials and Methods: All studies were performed on 12 female Wistar rats weighing 250±25 g. Isolated mesenteric ischemia and reperfusion were reproduced by ligation and subsequent removal of ligatures from three segmental arteries in the ileum. The saturation and activity of respiratory enzymes were studied by hyperspectral imaging and biofluorescence.

Results and Discussion: For the first time, we investigated the effect of L-norvaline at a dose of 15 mg/kg on tissue saturation and the activity of respiratory chain enzymes in ischemic and reperfusion injury of the small intestine. The study revealed an increase in the level of tissue saturation and NADH activity against the background of L-norvaline administration during ischemia, in the absence of a significant effect on saturation during reperfusion during the restoration of NADH activity.

Conclusion: The arginase inhibitor L-norvaline has a protective effect in ischemic and reperfusion injuries of the small intestine.

Graphical Abstract

Keywords:

ischemic and reperfusion injuries of the small intestine, L-norvaline

References

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Author Contribution

Sergey A. Alekhin, Kursk State Medical University

PhD in Medicine, Associate Professor, Associate Professor of the Department of Surgery №2, Kursk State Medical University, Kursk, Russia; e-mail: s_alehin@mail.ru; ORCID ID: https://orcid.org/0000-0003-1429-7362. Planning the experiments, analyzing the literature and interpreting the data.

Elena N. Bezhina , Belgorod State National Research University

Research assistant of the Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University, Belgorod, Russia; e-mail: elena_bejina@mail.ru; ORCID ID: https://orcid.org/0000-0003-3727-792X. The administration of the drugs to the animals and the modeling of acute intestinal ischemia.

Andrey V. Dunaev, Orel State University named after I. S. Turgenev

Doctor Habil. of Technical Sciences, Associate Professor, Professor of the Department of Instrument Engineering, Metrology and Certification, Orel State University named after I. S. Turgenev, Orel, Russia; e-mail: dunaev@bmecenter.ru; ORCID ID: https://orcid.org/0000-0003-4431-6288. Analyzing the literature and interpreting the data.

Dmitry P. Nazarenko, Kursk State Medical University

Doctor Habil. of Medical Sciences, Full Professor, Professor of the Department of Surgery №2, Kursk State Medical University, Kursk, Russia; e-mail: nazarenkodp003@yandex.ru; ORCID ID: https://orcid.org/0000-0002-2007-6825. Analyzing the literature and interpreting the data.

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Published

09-12-2025

How to Cite

Alekhin SA, Bezhina EN, Dunaev AV, Nazarenko DP (2025) First discovered effect of L-norvaline on tissue saturation and the activity of respiratory chain enzymes in ischemic and reperfusion injury of the small intestine. Research Results in Pharmacology 11(4): 38–41. https://doi.org/10.18413/rrpharmacology.11.861

Issue

Vol. 11 No. 4 (2025)

Section

Short Communication

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Copyright (c) 2025 Alekhin SA, Bezhina EN, Dunaev AV, Nazarenko DP

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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