The antiplatelet-antithrombotic effect and antioxidant activity of the Phlojodicarpus sibiricus extract
Авторы
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Sergey M. Gulyaev
Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences
https://orcid.org/0000-0001-8412-5799
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Ekaterina Z. Urbanova
Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences
https://orcid.org/0009-0003-2784-0894
- Tatyana A. Turtueva Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences;
DOI:
https://doi.org/10.18413/rrpharmacology.12.1107Аннотация
Introduction: Cardiovascular disease and ischemic stroke remain the leading causes of death and disability worldwide. Phlojodicarpus sibiricus has a wide range of pharmacological properties due to its content of coumarins. The aim of this study was to determine the antiplatelet effect and antioxidant activity of Phlojodicarpus sibiricus extract (P. sibiricus).
Material and Methods: A dry ethanol extract of P. sibiricus was used in the experiments. The antiplatelet activity of P. sibiricus was assessed using in vitro test. The effect of the extract on haemostasis was evaluated in models of blood coagulation and chemically induced vascular thrombosis. The experiments were conducted on 86 Wistar rats weighing 180-220 g, divided into groups: Group 1 – Vehicle, groups 2-4 – rats receiving P. sibiricus at doses of 50, 100, and 200 mg/kg, respectively; groups 5-6 – rats treated with aspirin (30 mg/kg) and heparin (200 U/kg), respectively. The antioxidant activity of the extract was assessed in vitro using the DPPH assay and the Fe2+ binding model.
Results: P. sibiricus demonstrated antiplatelet activity (in concentration 50 – 200 µg/mL) in vitro. Administration of P. sibiricus to rats at doses of 50, 100 and 200 mg/kg increased the duration of bleeding by 2 times and suppressed thrombus formation by 52.6% compared to the control.
Conclusion: P. sibiricus exerts an antiplatelet effect at doses of 50–200 mg/kg due to the combined antiplatelet and antioxidant effects of coumarins.
Графическая аннотация
Ключевые слова:
Phlojodicarpus sibiricus, сoumarins, dihydrosamidine, visnadin, antiplatelet activity, antioxidant activityБиблиографические ссылки
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