Effect of some compounds from the series of derivatives of cyanothioacetamide with analgesic activity on liver function parameters of rats with chronic administration of paracetamol against the background of alcohol load

Abstract

Introduction: Derivatives of 1,4-dihydropyridines from a series of α-cyanothioacetamide derivatives are a potentially promising class of compounds with analgesic activity.

Materials and methods: The experiment was carried out on 64 white male rats weighing 220-250 g. The rats were divided into 8 groups: intact, control, comparison (thiotriazoline 50 mg/kg) and 5 experimental groups (derivatives of 1.4-dihydropyridine and thiadiazine 5 mg/kg). Acute paracetamol-alcohol liver damage was modeled after two weeks of taking 1 mL of 40⁰ ethanol and paracetamol at a dose of 500 mg/kg.

Results: The total bilirubin level in the control group, compared to that in the intact, increased by 2.88 times, direct bilirubin – by 37.8%, ALT – by 38.85%, AST – by 51.54%, and alkaline phosphatase – by 163%. In the reference group, compared to the intact, the ALT level increased by 44.76%, AST – by 18.11%, alkaline phosphatase – by 19.4%, and AST reduced by 22.06%. In the Mar-014 group, direct bilirubin reduced by 42.7%. In the CV-150 compound group, total bilirubin reduced by 51.6%, AST – by 18.64%, and alkaline phosphatase – by 272.4%. In the TD-0331 group, the AST index increased by 35.7%, whereas alkaline phosphatase decreased by 43.1%. In the CV-131compound group, when compared with the control group, total bilirubin reduced by 75.3%, direct bilirubin – by 10.1%, and AST – by 12%. In the d02-123 group, no indicators exceeded the values of rats in the intact group.

Conclusion: The most pronounced hepatoprotective properties were shown by the compound with the code d02-123.

Graphical Abstract