Pharmacological preconditioning by recombinant erythropoietin as the possibility of increasing the stability of tissue of the retina to reperfusion ischemia in experiment

Authors

  • Anna Sergeevna Shabelnikova Belgorod National Research University
  • Anna A. Peresypkina Belgorod National Research University
  • Viktoriya O. Gubareva Perinatal center of St. Joasaph
  • Elena Aleksandrovna Levkova OGBUZ "Yakovlevskaya CRH"
  • Alexandr A. Dolzhikov Belgorod National Research University
  • Sergej Borisovich Nikolaev St. Joasaph Belgorod Regional Clinical Hospital
  • Aleksandr A. Stepchenko Kursk State Medical University

Abstract

Ischemic injury was formed in laboratory rats by applying mechanical pressure (110 mm Hg) to the anterior eye chamber for 30 minutes. The experiment revealed that the recombinant erythropoietin (50 IU/kg) prevents the development of degenerative changes in retinal layers caused by ischemic injury. Intensity of the protective effect of erythropoietin on the retina of rats was assessed after 1 hour and 72 hours of reperfusion after pathology simulation. The observed protective effect of erythropoietin with the development of ischemia was confirmed by laser Doppler flowmetry, electroretinography and histomorphometry. Identification and use of pharmacological agents with the preconditioning effect may be a new approach in the correction and prevention of retinal ischemia, which is the leading element in the pathogenesis of a number of ophtalmic pathologies. The possibility of pharmacological preconditioning of ischemic lesions of the retina with erythropoietin is essential for the development of anti-ischemic agents for the treatment and prevention of ischemic ophthalmic pathologies.

Keywords:

retinal ischemia-reperfusion, pharmacological preconditioning, erythropoietin, ATPdependent potassium channels

Author Contribution

Anna Sergeevna Shabelnikova, Belgorod National Research University

Assistant Lecturer of the Department of Pharmacology of Belgorod State National Research University, Belgorod, Russia

Anna A. Peresypkina, Belgorod National Research University

PhD in Biological Sciences, Senior Lecturer of the Department of Pharmacology of Belgorod State National Research University, Belgorod,  Russia 

Viktoriya O. Gubareva, Perinatal center of St. Joasaph

Ophthalmologist of the Pathology department of newborns and premature infants of the perinatal center of St. Joasaph, Belgorod, Russia

Elena Aleksandrovna Levkova, OGBUZ "Yakovlevskaya CRH"

ophthalmologist of OGBUZ "Yakovlevskaya CRH", Stroitel, Yakovlevsky region, Russia 

Alexandr A. Dolzhikov, Belgorod National Research University

Doctor of Medical Sciences, Professor Department of histology of Medical Institute of Belgorod State National Research University, Belgorod, Russia

Sergej Borisovich Nikolaev, St. Joasaph Belgorod Regional Clinical Hospital

Doctor of Medical Sciences, St. Joasaph Belgorod Regional Clinical Hospital, Belgorod, Russia.

Aleksandr A. Stepchenko, Kursk State Medical University

Doctor of Medical Sciences, Kursk State Medical University, Kursk, Russia.  

Downloads

Published

31-03-2016

How to Cite

Shabelnikova AS, Peresypkina AA, Gubareva VO, Levkova EA, Dolzhikov AA, Nikolaev SB, Stepchenko AA (2016) Pharmacological preconditioning by recombinant erythropoietin as the possibility of increasing the stability of tissue of the retina to reperfusion ischemia in experiment. Research Results in Pharmacology 2(1): 25–29

Issue

Section

Review article

Most read articles by the same author(s)

1 2 > >>