A novel view of the problem of Osteoarthritis in experimental rat model

Authors

  • Viktor A. Palikov Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ORCID logo https://orcid.org/0000-0003-2989-4477
  • Yuliya A. Palikova Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ORCID logo https://orcid.org/0000-0001-9547-0686
  • Natalya A. Borozdina Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences ORCID logo https://orcid.org/0000-0002-2320-655X
  • Elena N. Nesmeyanova Volgograd State University
  • Pavel A. Rudenko Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences
  • Vitalij A. Kazakov Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences
  • Elena A. Kalabina Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
  • Mihail V. Bukatin Volgograd State Medical University
  • Tatyana Yu. Zharmukhamedova Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
  • Oksana N. Khokhlova Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
  • Igor A. Dyachenko Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences ORCID logo https://orcid.org/0000-0002-3053-2804

DOI:

https://doi.org/10.3897/rrpharmacology.6.51772

Abstract

Introduction: The article presents the results of the functional tests to improve the assessment of MIA-induced osteo­arthritis development and the effectiveness of NSAID therapy.

Materials and methods: In the study, 26 male SD rats were used. MIA-induced osteoarthritis was simulated in the right knee joint. After an intra-articular injection of MIA, the animals were treated with ibuprofen and meloxicam. Pain assessment was studied in the following functional tests: incapacitance (hind limb weight bearing) test, von Frey test (mechanical allodynia), grip strength test, and knee diameter measurement. At the end of the study, a histological analysis of the knee joint was performed.

Results and discussion: An intra-articular MIA injection reduced 1.5 times the paw withdrawal threshold. In the rats that suffered MIA-induced osteoarthritis, the difference between the diameters of the intact and injected joints was 1.05 mm, compared to 0.03 mm difference in the control group. Hind limb weight bearing asymmetry was 89.5% when simulating MIA-induced osteoarthritis. The muscular hind limb grip strength in rats with MIA-induced osteoarthritis was significantly reduced on 3rd and 7th days after simulating osteoarthritis. Ibuprofen and meloxicam showed signif­icant efficacy in all the above tests, although ibuprofen effectiveness was more pronounced than that of meloxicam.

Conclusion: The following functional tests were identified as the most significant and sufficient to assess the devel­opment of MIA-induced osteoarthritis and analgesic efficacy of NSAIDs: incapacitance test, allodynia test (von Frey filaments), measurement of hind limb grip strength and measurement of the diameter of the inflamed knee joint. The histological analysis made it possible to confirm the correspondence of the physiological response and pathological changes in the knee joint.

Keywords:

in vivo model, monoiodoacetic acid (MIA), osteoarthritis, pain assessment, rat

Author Contribution

Viktor A. Palikov, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Researcher, Laboratory of Biological Testing.

Yuliya A. Palikova, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Researcher, Laboratory of Biological Testing.

Natalya A. Borozdina, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Junior researcher, Laboratory of Biological Testing.

Elena N. Nesmeyanova, Volgograd State University

Assistant.

Pavel A. Rudenko, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences

Doctor of Veterinary Sciences, senior researcher, Laboratory of Biological Testing.

Vitalij A. Kazakov, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences

Doctor habilitated in Medicine, senior researcher, Laboratory of Biological Testing.

Elena A. Kalabina, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Junior researcher, Laboratory of Biological Testing.

Mihail V. Bukatin, Volgograd State Medical University

PhD in Medical Sciences.

Tatyana Yu. Zharmukhamedova, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

PhD in Biological Sciences, researcher, Laboratory of Biological Testing.

Oksana N. Khokhlova, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

PhD in Biological Sciences, senior researcher, Laboratory of Biological Testing.

Igor A. Dyachenko, Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Pushchino State Institute of Natural Sciences

PhD in Biological Sciences, senior researcher, Laboratory of Biological Testing.

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Published

19-06-2020

How to Cite

Palikov VA, Palikova YA, Borozdina NA, Nesmeyanova EN, Rudenko PA, Kazakov VA, Kalabina EA, Bukatin MV, Zharmukhamedova TY, Khokhlova ON, Dyachenko IA (2020) A novel view of the problem of Osteoarthritis in experimental rat model. Research Results in Pharmacology 6(2): 19–25. https://doi.org/10.3897/rrpharmacology.6.51772

Issue

Section

Review article