Neuroprotective activity of Styphnolobium japonicum fruit extract in cerebral ischemia-reperfusion injury

Authors

DOI:

https://doi.org/10.18413/rrpharmacology.11.797

Abstract

Introduction: Recently, there has been a growing interest in the study of flavonoids with neuroprotective activity. Fruits of the Styphnolobium japonicum can be used as the source of such flavonoids. The research aim was to determine the neuroprotective effect of Styphnolobium japonicum fruit extract (SFE) in rats with cerebral ischemia-reperfusion.

Materials and Methods: In the experiment, Wistar rats were used, divided into groups: 1 – sham operated rats, water; 2 – control, water + cerebral ischemia-reperfusion (I-R); 3 – rats treated with SFE at a dose of 200 mg/kg + I-R; 4 – rats treated with ginkgo biloba extract (GBE) at a dose of 50 mg/kg + I-R. The I-R model was reproduced in rats by the occlusion of the common carotid arteries with hypotension. The SFE effect on the neurological status of animals and their behavior was determined using neurological and ethological tests such as an open field (OF) and an elevated plus maze (EPM) ones. The SFE effect on the brain morphofunctional state in the rats with ischemia-reperfusion was determined by the pathomorphological examination.

Results: The neurological deficit in the test groups was 3 times lower than the control values, the indicators of motor activity in the OF test were 2 times higher; the level of anxiety in rats in the EPM test was lower: the number of visits to the open arms of the maze and the duration of stay there were 3.8 times and 10 times greater, respectively, compared with those in the control. In the test group rats, the proportions of regressive forms of neurons in the cortex and hippocampus were lower than in the control rats. The dominant component here is sophoraflavonoloside.

Conclusion: SFE has a neuroprotective effect on rats with cerebral ischemia-reperfusion. The SFE neuroprotective effect may presumably be due to the activity of kaempferol glycosides, the dominant of which is sophoraflavonoloside.

Graphical Abstract

Keywords:

Styphnolobium japonicum, sophoraflavonoloside, kaempferol glycosides, cerebral ischemia-reperfusion, neuroprotection, morphology

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Author Contribution

Sergey M. Gulyaev, Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences

Candidate of Medical Sciences, Senior Researcher at the Laboratory of Experimental Pharmacology, the Institute of General and Experimental Biology of the Siberian Branch of the Russian Academy of Sciences, Ulan-Udeh, Russia; e-mail: s-gulyaev@inbox.ru; ORCID ID: https://orcid.org/0000-0001-8412-5799. The author contributed to the concept development, performing pharmacological studies, and conducting the analysis of the data obtained.

Olga L. Saybel, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Doctor Habil. of Pharmaceutical Sciences, Head of the Center for Chemistry and Pharmaceutical Technology, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia; e-mail: saybel@vilarnii.ru; ORCID ID: https://orcid.org/0000-0001-8059-5064. The author contributed to the research planning, data analysis, writing and editing the manuscript.

Andrey I. Radimich, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Senior Researcher at the Department of Chemistry of Natural Compounds, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia; e-mail: vilarnii.radimich@mail.ru; ORCID ID:https://orcid.org/0000-0002-1139-8902. The author contributed to the sophoraflavonoloside isolation and identification.

Tamara D. Dargaeva, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants

Doctor Habil. of Pharmaceutical Sciences, Professor, Chief Employee of the Department of Chemistry of Natural Compounds, All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia; e-mail: olster@mail.ru; ORCID ID: https://orcid.org/0000-0002-0722-9479. The author contributed to the research planning, writing and editing the manuscript.

Tamara D. Rendyuk, Sechenov First Moscow State Medical University (Sechenov University)

Candidate of Pharmaceutical Sciences, Associate Professor of the Department of Pharmaceutical Natural Sciences, Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia; e-mail: aramat_17@mail.ru; ORCID ID: https://orcid.org/0000-0002-0359-3847. The author contributed to the collection and analysis of raw materials.

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Published

24-12-2025

How to Cite

Gulyaev SM, Saybel OL, Radimich AI, Dargaeva TD, Rendyuk TD (2025) Neuroprotective activity of Styphnolobium japonicum fruit extract in cerebral ischemia-reperfusion injury. Research Results in Pharmacology 11(4): 131–139. https://doi.org/10.18413/rrpharmacology.11.797

Issue

Vol. 11 No. 4 (2025)

Section

Experimental Pharmacology

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Copyright (c) 2025 Gulyaev SM, Saybel OL, Radimich AI, Dargaeva TD, Rendyuk TD

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