Анализ нейропротекторной активности вторичных метаболитов мирта в модели ишемии-реперфузии у крыс: суммарная электрическая активность и неврологический дефицит

Авторы

DOI:

https://doi.org/10.18413/rrpharmacology.12.1108

Аннотация

Введение: Целью исследования было изучение влияния вторичных метаболитов мирта (гидролата, сиропа I и сиропа II) на общую электрическую активность головного мозга и выраженность неврологического дефицита у крыс в условиях моделируемой ишемии-реперфузии.

Материалы и методы: Модель ишемии-реперфузии воспроизводили на 60 самцах крыс линии Wistar путем 30-минутной окклюзии левой общей сонной артерии с последующим восстановлением кровотока. Животных разделили на шесть групп (n=10): группа ложнооперированных животных (ЛО); группа контроля ишемии (КИ); группа сравнения (препарат «Тиоцетам»); а также экспериментальные группы, получавшие внутрижелудочно гидролат мирта (Hydrolate), сироп I (Syrup I) и сироп II (Syrup II) в дозе 2,5 мл/кг ежедневно в течение 21 дня.

Результаты: Ишемия вызывала стойкое снижение амплитуд ЭЭГ (на 40–61%), перестройку спектра с преобладанием дельта- (+47–63%) и тета-ритмов (+41–119%) и подавлением альфа-ритма (–54–75%), а также выраженный неврологический дефицит. Гидролат оказывал умеренный эффект в раннем периоде, однако к 21-му дню амплитуды снизились на 12–14% ниже уровней группы контроля ишемии (КИ). Сироп I способствовал восстановлению амплитуд (+29–82%), спектральных характеристик (альфа-ритм +151–345%, дельта-ритм –36%) и неврологического статуса к 7–14-му дню. Сироп II устранил неврологический дефицит уже ко 2-му дню (0,08 балла по шкале Бедерсона против 1,67 в группе КИ); к 7-му дню неврологический дефицит был полностью устранен, при этом амплитуды ЭЭГ возросли на 17–63%, а выраженность альфа-ритма — на 104–272%. Обсуждение: Выявленные различия в нейропротекторной активности трех композиций вторичных метаболитов мирта коррелируют с их химическим составом. По интенсивности нейропротекторного действия вторичные метаболиты мирта расположились в следующем порядке: гидролат < сироп I ≈ тиоцетам < сироп II.

Заключение: Вторичные метаболиты мирта, в частности сироп II с высоким содержанием флавоноидов, оказывают комплексное нейропротекторное действие, которое проявляется в согласованном восстановлении электрофизиологических и поведенческих показателей.

 

Графическая аннотация

Ключевые слова:

ишемия-реперфузия, мирт обыкновенный, вторичные метаболиты, электроэнцефалография, неврологический дефицит, мирицетин, фенольные кислоты, нейропротекция, крысы

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Вклад авторов

Marina Yu. Ravaeva, V.I. Vernadsky Crimean Federal University

Candidate of Biological Sciences, Associate Professor of the Department of Human and Animal Physiology and Biophysics of the Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol, e-mail: ravaevam@yandex.ru; ORCID ID: https://orcid.org/0000-0002-6081-1628. Developing the concept; forming the idea and focus of the study; developing and designing the methodology, and formulating key goals and objectives.

Eva S. Yan, V.I. Vernadsky Crimean Federal University

2nd year Master’s student, records manager of the Directorate of the Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol; e-mail: vigman@bk.ru. Conducting the study; analyzing and interpreting the data; and participating in the scientific design.

Irina A. Roslyakova, V.I. Vernadsky Crimean Federal University

1st-year Master’s student, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol; e-mail: iravaviliina@mail.ru. Conducting the study; analyzing and interpreting the data; and participating in the scientific design.

Maria V. Nagorskaya, V.I. Vernadsky Crimean Federal University

Head of laboratories, 3-year postgraduate student in specialty 1.5.5. Human and Animal Physiology, Department of Human and Animal Physiology and Biophysics, Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol; e-mail: zaygolnikova@mail.ru; ORCID ID: https://orcid.org/0009-0002-0528-2791. Conducting the study; analyzing and interpreting the data; and participating in the scientific design.

Elena N. Chuyan, V.I. Vernadsky Crimean Federal University

Doctor Habil. of Biological Sciences, Professor, Head of the Department of Human and Animal Physiology and Biophysics at the Institute of Biochemical Technologies, Ecology and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol; e-mail: elena-chuyan@rambler.ru; ORCID ID: https://orcid.org/0000-0001-6240-2732. Developing the concept, key goals and objectives; interpreting data; drafting and critically revising the manuscript; participating in scientific design; being responsible for all aspects of the work, the integrity of all parts of the article, and its final version.

Anait V. Zelenskaya, Kuban State Medical University

PhD in Medicine, Associate Professor, Pharmacology Department, Kuban State Medical University, Krasnodar, Russian Federation; e-mail: anait_06@mail.ru; ORCID ID: https://orcid.org/0000-0001-9512-2526. Analyzing and interpreting the study results; preparing and editing the article, and participating in scientific design.

Andrey I. Sidyakin, y, V.I. Vernadsky Crimean Federal University

  • PhD in Biology, Associate Professor, Department of Botany, Plant Physiology, and Biotechnology, Institute of Biochemical Technologies, Ecology, and Pharmacy, V.I. Vernadsky Crimean Federal University, Russian Federation, Simferopol; e-mail: acid2302@mail.ru; ORCID ID: https://orcid.org/0000-0002-5125-7514. Procurement of myrtle samples, preparation of substances for administration, analysis of chemical composition developing the concept, key goals and objectives; interpreting data; drafting and critically revising the manuscript; participating in scientific design; being responsible for all aspects of the work, the integrity of all parts of the article, and its final version.

Ekaterian Yu. Bakova, Federal State Budgetary Institution “Order of the Red Banner of Labor Nikitsky Botanical Gardens – National Scientific Center of the Russian Academy of Sciences”

unior researcher, Laboratory of Human Phytorehabilitation, Federal State Budgetary Institution “Order of the Red Banner of Labor Nikitsky Botanical Gardens – National Scientific Center of the Russian Academy of Sciences”, Russian Federation, Yalta; e-mail: e_bakova@bk.ru; ORCID ID: https://orcid.org/0000-0003-4995-8044. Procurement of myrtle samples, preparation of substances for administration, and analysis of chemical composition.

Загрузки

Опубликован

30.09.2026

Как цитировать

Ravaeva MY, Yan ES, Roslyakova IA, Nagorskaya MV, Chuyan EN, Zelenskaya AV, Sidyakin AI, Bakova EY (2026) Analysis of the neuroprotective activity of myrtle secondary metabolites in an ischemia-reperfusion model in rats: Overall electrical activity and neurological deficit. Research Results in Pharmacology 12(3): 129–144. https://doi.org/10.18413/rrpharmacology.12.1108

Выпуск

Том 12 № 3 (2026)

Раздел

Экспериментальная фармакология

Лицензия

Copyright (c) 2026 Ravaeva MYu, Yan ES, Roslyakova IA, Nagorskaya MV, Chuyan EN, Zelenskaya AV, Sidyakin AI, Bakova EYu

Лицензия Creative Commons

Это произведение доступно по лицензии Creative Commons «Attribution» («Атрибуция») 4.0 Всемирная.

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