Efficacy of liposomal dosage forms and hyperosmolar salines in experimental pharmacotherapy of acute lung injury

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DOI:

https://doi.org/10.3897/rrpharmacology.5.35529

Аннотация

Introduction: Hypertonic sodium chloride solutions and liposomal drugs with pulmotropic effect are of great interest for the treatment of acute lung injury (ALI). The results of the studies on the efficacy of hypertonic solutions and lipo[1]somes in ALI treatment are currently controversial.

Materials and methods: For the experiment, liposomes with dexamethasone, N-acetylcysteine (NAC), aprotinin and dye Cyanine-7 (Cy-7) were obtained. A liposome analysis was performed by means of spectrophotometry. ALI was modeled in rats by the administration of the damaging agents into the trachea. The experimental agents were injected once intravenously after the modeling of ALI. For experimental therapy used liposomal agents, 7.5% hypertonic saline (HS) and HyperHAES solutions in the respective groups. The efficacy of the therapy was assessed by the survival of animals, functional indicators of the cardiovascular and respiratory systems, and by the lung-body ratio. The biodistri[1]bution of liposomes after intravenous administration was investigated in mice through using a fluorescent dye Cy-7. The biodistribution of liposomes with Cy-7 was assessed using bioimaging according to the fluorescence intensity of internal organs (lungs, liver, and kidneys) and blood, expressed as dye concentration according to the calibration de[1]pendence of dye concentrarion on fluorescence intensity.

Results and discussion: All the studied liposomal drugs were effective for the pharmacological correction of ALI. Hypertonic solutions, unlike liposomal drugs, were less likely to prevent the development of pulmonary edema. All the studied therapeutic agents increased the survival rate of the laboratory animals with ALI. The most effective experimen[1]tal agent was liposomal dexamethasone. The use of drugs in form of simple liposomes with average diameter of 350 nm provided for a higher concentration of the drug in the lungs within the first 40 minutes after intravenous administration.

Conclusion: Intravenous administration of liposomal forms is promising for the pharmacotherapy of acute lung injury.

Ключевые слова:

N-acetylcysteine, acute lung injury, aprotinin, Cyanine-7, dexamethasone, HyperHAES, hypertonic saline, liposomes

Вклад авторов

Oleg A. Kulikov, National Research Ogarev Mordovia State University

Candidate of Medical Sciences PhD in Medicine, Associated Professor of the Department of Pharmacology and Clinical Pharmacology with a course of Pharmaceutical Technology.

Valentin P. Ageev, National Research Ogarev Mordovia State University

Postgraduate student of the Department of Pharmacology and Clinical Pharmacology with a
course of Pharmaceutical Technology.

Elena E. Marochkina, I.M. Sechenov First Moscow State Medical University

Elena E. Marochkina, Provisional pharmacist (intern), Department of Pharmacy Organization and Economics.

Irina S. Dolgacheva, I.M. Sechenov First Moscow State Medical University

Provisional pharmacist (intern), Department of Pharmacy Organization and Economics.

Olga V. Minayeva, I.M. Sechenov First Moscow State Medical University

Candidate of Medical Sciences, PhD in Medicine, Associated Professor of the Department of Anesthesiology and Rheumatology.

Vera I. Inchina, National Research Ogarev Mordovia State University

Doctor of Medical Sciences, Full Professor, Head of Department of Pharmacology and Clinical Pharmacology with a course of Pharmaceutical Technology.

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Опубликован

26.06.2019

Как цитировать

Kulikov OA, Ageev VP, Marochkina EE, Dolgacheva IS, Minayeva OV, Inchina VI (2019) Efficacy of liposomal dosage forms and hyperosmolar salines in experimental pharmacotherapy of acute lung injury. Research Results in Pharmacology 5(2): 23–41. https://doi.org/10.3897/rrpharmacology.5.35529

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