Metal-containing taurine compounds protect rat’s brain in reperfusion-induced injury

Авторы

  • Elena V. Semeleva Ogarev Mordovia State University
  • Ekaterina V. Blinova Sechenov University ORCID logo https://orcid.org/0000-0003-0050-0251
  • Andrey V. Zaborovsky A.I.Yevdokimov Moscow State University of Medicine and Dentistry
  • Irina A. Gromova Ogarev Mordovia State University
  • Asliddin S. Shukurov Sechenov University
  • Dmirty S. Blinov All-Union Research Center for Safety of Biological Active Substances ORCID logo https://orcid.org/0000-0002-8385-4356
  • Egor A. Turovsky Institute of Cell Biophysics, Russian Academy of Sciences
  • Olga V. Vasilkina Ogarev Mordovia State University
  • Elena G. Lobanova A.I.Yevdokimov Moscow State University of Medicine and Dentistry
  • Elena A. Samishina Ogarev Mordovia State University
  • Yan A. Mazov Ogarev Mordovia State University
  • Alexey I. Sokolov Sechenov University
  • Yulia V. Dergunova Ogarev Mordovia State University

DOI:

https://doi.org/10.3897/rrpharmacology.6.59857

Аннотация

Introduction: The study aim was to explore a neuroprotective action of magnesium (LKhT-317) and zinc (LKhT-318) taurine salts on experimental models of reperfusion brain damage in rats and cell culture.

Materials and methods: The study was performed on male Sprague Dawley rats, and rat’s hippocampal mixed neu­roglial cell culture. Magnesium- (LKhT-317) and zinc-containing (LKhT-318) derivatives of taurine were studied. Reperfusion brain damage was induced 30 min after intraluminal cerebral middle artery occlusion. Severity of the injury was assessed by local blood flowmetry, neurological symptoms scaling and brain tissue staining. Levels of IL-1b, IL-10 and TNF-alpha in tissue were determined by qualitative ELISA. Caspase-3 and Bcl-2 expressions were detected by IHC. Neurons survival was assessed by cytochemistry. Cellular calcium responses were detected by fluorescent microscopy of Fura-2-containig cells.

Results and discussion: Metal-containing taurine derivatives – LKhT-317 and LKhT-318 – demonstrated a sufficient neuroprotective property in rats with a reperfusion-induced brain injury. Both derivatives effectively prevented severity of the animals’ brain damage, motor deficiency, reduction of microvascular perfusion, and proinflammatory cytokines production. Magnesium-containing compound LKhT-317 was comparatively more effective than zinc-containing one. LKhT-317 possessed an anti-apoptotic action in vivo, and protected neurons from OGD-mediated cell death in mixed hippocampal culture. The aforementioned actions may be associated with an LKhT-317 inhibitory effect on NMDA-in­duced cellular Ca2+ response and, therefore, the anti-excitotoxic property of the compound.

Conclusion: Magnesium- and zinc-containing taurine derivatives may be considered as promising neuroprotectors in the reperfusion-induced brain injury.

Ключевые слова:

brain injury, metal-containing taurine derivatives, reperfusion, cerebral middle artery, Ca2 response, hippocampal mixed cell culture, rats

Вклад авторов

Elena V. Semeleva, Ogarev Mordovia State University

Candidate of Medical Sciences, Associate Professor, Department of Public Health.

Ekaterina V. Blinova, Sechenov University

Doctor Habil. of Medical Sciences, Professor of the Department of Operative Surgery and Clinical Anatomy.

Andrey V. Zaborovsky, A.I.Yevdokimov Moscow State University of Medicine and Dentistry

Doctor Habil. of Medical Sciences, Head of Pharmacology Department.

Irina A. Gromova, Ogarev Mordovia State University

Postgraduate student, Department of Pharmacology.

Asliddin S. Shukurov, Sechenov University

Postgraduate student, Department of the Operative Surgery.

Dmirty S. Blinov, All-Union Research Center for Safety of Biological Active Substances

Doctor Habil. of Medical Sciences, Principal Researcher, Pharmacology Laboratory.

Egor A. Turovsky, Institute of Cell Biophysics, Russian Academy of Sciences

Candidate of Medical Sciences, Associate Professor.

Olga V. Vasilkina, Ogarev Mordovia State University

Candidate of Medical Sciences, Assistant Professor, Department of Hospital Surgery.

Elena G. Lobanova, A.I.Yevdokimov Moscow State University of Medicine and Dentistry

Doctor Habil. of Medical Sciences, Professor of Pharmacology Department.

Elena A. Samishina, Ogarev Mordovia State University

Candidate of Medical Sciences, Senior Researcher, Laboratory of Specific Pharmacological Activity.

Yan A. Mazov, Ogarev Mordovia State University

Postgraduate student, Department of faculty surgery.

Alexey I. Sokolov, Sechenov University

Postgraduate student, Department of the Operative Surgery.

Yulia V. Dergunova, Ogarev Mordovia State University

Postgraduate student, Department of faculty surgery.

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Опубликован

20.11.2020

Как цитировать

Semeleva EV, Blinova EV, Zaborovsky AV, Gromova IA, Shukurov AS, Blinov DS, Turovsky EA, Vasilkina OV, Lobanova EG, Samishina EA, Mazov YA, Sokolov AI, Dergunova YV (2020) Metal-containing taurine compounds protect rat’s brain in reperfusion-induced injury. Research Results in Pharmacology 6(4): 43–49. https://doi.org/10.3897/rrpharmacology.6.59857

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