Study to elucidate the pharmacological activity of retinalamin in a rat model of ischemic retinopathy

Authors

DOI:

https://doi.org/10.3897/rrpharmacology.7.67390

Abstract

Introduction: Over the past few years, the incidence of retinal ischemic disorders has been increasing, due to a rising prevalence of such socially burdensome diseases as diabetes and hypertension, which ultimately lead to ocular vascular pathology. The identification of new treatment options that would prevent retinal neuron death is a crucial task of modern pharmacology.

Materials and methods: The research was carried out on male Wistar rats. Retinopathy was modeled by inducing a 30-min ischemic episode, with a 72-hour period of reperfusion and subsequent administration of Retinalamin and Emoxypine for 10 days. The effectiveness of the drugs was evaluated by electroretinographic, ophthalmoscopic and morphological assessments.

Results and discussion: On Day 14 of the experiment, a dose-dependent preservation of the electroretinogram b-wave/a-wave amplitude ratio was observed in the animals treated with Retinalamin depending on a dose (1.39±0.06, 1.46±0.03 and 1.49±0.04 in low (0.214 mg/kg), medium (0.428 mg/kg) and high (0.857 mg/kg) Retinalamin dose groups, respectively). The ophthalmoscopic picture of the fundus oculi also improved following the treatment with Retinalamin (1.42, 1.69 and 1.90 times lower ophthalmoscopic scores compared to placebo-treated animals in low, medium and high dose groups, respectively). The morphologic “coefficient of change” applied to ganglion cell layer was 2.2, 1.7 and 1.6 points in low, medium and high dose Retinalamin groups, respectively. These results are significantly different from both intact and placebo group (p<0.05). Based on the aforementioned experimental findings, we conclude that Retinalamin has a retinoprotective effect and is superior to the drug of comparison (Emoxypine).

Conclusion: The greatest neuroprotective effects were shown in the groups receiving Retinalamin. In these groups, the ERG b-wave/a-wave amplitude ratio was preserved, the ophthalmoscopic picture was less pathologic and retinal morphology features were close to those of the intact retina.

Keywords:

ganglion cell layer, inner nuclear layer, retina, Retinalamin

Author Contribution

Anna S. Pobeda, Belgorod State National Research University

PhD in Biological Sciences, Associate Professor of the Department of Pharmacology and Clinical Pharmacology.

Anna V. Kalatanova, Pharm-Holding CJSC

Scientific officer of the Pharmacology Department

Daria A. Abashev, Pharm-Holding CJSC

Medical writer of the Department of Early Phase of Clinical Trials.

Aleksandr A. Dolzhikov, Belgorod State National Research University

PhD in Medical Sciences, Professor of the Department of Human Anatomy and Histology

Nikolai V. Solovev, Belgorod State National Research University

PhD Student majoring in Pharmacology, Clinical Pharmacology

Kristina V. Shchurovskaya, Belgorod State National Research University

PhD Student majoring in Pharmacology, Clinical Pharmacology

Sofia S. Chernyaeva, Belgorod State National Research University

PhD Student majoring in Pharmacology, Clinical Pharmacology

Inga M. Kolesnik, Belgorod State National Research University

PhD in Medical Sciences, Associate Professor, Department of Pharmacology and Clinical Pharmacology

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Published

29-06-2021

How to Cite

Pobeda AS, Kalatanova AV, Abashev DA, Dolzhikov AA, Solovev NV, Shchurovskaya KV, Chernyaeva SS, Kolesnik IM (2021) Study to elucidate the pharmacological activity of retinalamin in a rat model of ischemic retinopathy. Research Results in Pharmacology 7(2): 39–48. https://doi.org/10.3897/rrpharmacology.7.67390

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Section

Review article

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