L-NAME-induced Preeclampsia: correction of functional disorders of the hemostasis system with Resveratrol and Nicorandil

Authors

DOI:

https://doi.org/10.3897/rrpharmacology.5.35316

Abstract

Introduction. Preeclampsia is a formidable disease of the second half of pregnancy, leading to severe complications, including disability and even death. Many authors have recognized the correlation between the severity of preeclampsia and the degree of disturbances in the hemostasis system. In this regard, the objective of this study was to assess inhibition of platelet aggregation and the possibility of its correction with resverаtrol and nicorandil.

Materials and methods. The study was performed on 250 mature white Wistar female rats weighing 250–300 g. The platelet aggregation induced by ADP, collagen, ristomycin, adrenaline was determined, as well as PTT, TT, aPTT, fibrinogen, and the clotting time.

Results and discussion. Introduction of resverаtrol and nicorandil resulted in a decrease in thrombocyte aggregation capacity from 53.8 ± 2.60% to 22.1 ± 1.25% and 37.1 ± 1.79%, respectively, when using ADP as an inducer. The clotting time was from 841 ± 42 s up to 1135 ± 33 s and 1034 ± 26 s, respectively. In addition, there was an increase intemporal parameters of plasma-coagulation hemostasis and a decrease in plasma fibrinogen content. The use of gliben-clamide resulted in partial cancellation of the positive effects of resverаtrol and nicorandil, with an increase in platelet aggregation to 28.9 ± 1.8% and 43.9 ± 1.2% when using ADP as an inducer and a decrease in the thrombosis time to 988 ± 26 s and 950 ± 22 s, respectively.

Conclusion. In animals with experimental preeclampsia, there were disturbances in the hemostasis system, comparable to those in the clinical situation. The use of resverаtrol and nicorandil leads to a pronounced correction of hemostasis parameters. The positive effects of the studied pharmacological agents are mediated by several mechanisms, including K+ATP channels.K+ATP channels.

 

Keywords:

experimental preeclampsia, platelet aggregation, hemostasis, nicorandil, resverаtrol

Author Contribution

Elena G. Stupakova, Kursk State Medical University of the Ministry of Healthcare of the Russian Federation

Assistant, Department of Obstetrics and Gynecology, Faculty of Postgraduate Education.

Galina A. Lazareva, Kursk State Medical University of the Ministry of Healthcare of the Russian Federation

Doctor of Medical Sciences, Professor, Head of the Department of Obstetrics and Gynaecology, Faculty of Postgraduate Education.

Vladimir V. Gureev, Kursk State Medical University of the Ministry of Healthcare of the Russian Federation

Doctor of Medical Sciences, Professor of the Department of Pharmacology and Clinical Pharmacology.

Irina N. Dolzhikova, Kursk State Medical University of the Ministry of Healthcare of the Russian Federation

PhD of Biological Sciences, Associate Professor of the Department of Pharmacology and Clinical Pharmacology.

Lyudmila A. Zhilinkova, Kursk Academy of the Public and Municipal Service

PhD in Technical Sciences, Associate Professor, Department of Philosophy, Social, Legal and Natural Sciences.

Anastasia Gureeva, Kursk State Medical University of the Ministry of Healthcare of the Russian Federation

2nd-year student, Medical Faculty.

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Published

19-04-2019

How to Cite

Stupakova EG, Lazareva GA, Gureev VV, Dolzhikova IN, Zhilinkova LA, Gureeva A (2019) L-NAME-induced Preeclampsia: correction of functional disorders of the hemostasis system with Resveratrol and Nicorandil. Research Results in Pharmacology 5(2): 1–12. https://doi.org/10.3897/rrpharmacology.5.35316

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Review article

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