Genetically modified animals for use in biopharmacology: from research to production

Alexey V.  Deykin; Olesya V. Shcheblykina; Elena E. Povetka; Polina A. Golubinskaya; Vladimir M.  Pokrovskiy; Liliya V. Korokina; Olesya A. Vanchenko; Elena V. Kuzubova; Konstantin S. Trunov; Viktor V. Vasyutkin; Alexandra I. Radchenko; Anton P. Danilenko; Julia V. Stepenko; Indira S. Kochkarova; Veronika S. Belyaeva; Vladimir I. Yakushev

doi:10.3897/rrpharmacology.7.76685

Authors

Alexey V. Deykin Belgorod State National Research University https://orcid.org/0000-0001-9960-0863
Olesya V. Shcheblykina Belgorod State National Research University https://orcid.org/0000-0003-0346-9835
Elena E. Povetka Belgorod State National Research University https://orcid.org/0000-0003-4517-1041
Polina A. Golubinskaya Belgorod State National Research University https://orcid.org/0000-0002-1765-9042
Vladimir M. Pokrovskiy Belgorod State National Research University https://orcid.org/0000-0003-3138-2075
Liliya V. Korokina Belgorod State National Research University. https://orcid.org/0000-0002-4115-1564
Olesya A. Vanchenko Belgorod State National Research University
Elena V. Kuzubova Belgorod State National Research University https://orcid.org/0000-0003-2425-5027
Konstantin S. Trunov Belgorod State National Research University
Viktor V. Vasyutkin Belgorod State National Research University
Alexandra I. Radchenko Belgorod State National Research University https://orcid.org/0000-0002-4554-2116
Anton P. Danilenko Belgorod State National Research University
Julia V. Stepenko Belgorod State National Research University https://orcid.org/0000-0002-7414-7326
Indira S. Kochkarova Belgorod State National Research University
Veronika S. Belyaeva Belgorod State National Research University https://orcid.org/0000-0003-2941-0241
Vladimir I. Yakushev Belgorod State National Research University

DOI:

https://doi.org/10.3897/rrpharmacology.7.76685

Abstract

Introduction: In this review, the analysis of technologies for obtaining biologically active proteins from various sources is carried out, and the comparative analysis of technologies for creating producers of biologically active proteins is presented. Special attention is paid to genetically modified animals as bioreactors for the pharmaceutical industry of a new type. The necessity of improving the technology of development transgenic rabbit producers and creating a platform solution for the production of biological products is substantiated.

The advantages of using TrB for the production of recombinant proteins: The main advantages of using TrB are the low cost of obtaining valuable complex therapeutic human proteins in readily accessible fluids, their greater safety relative to proteins isolated directly from human blood, and the greater safety of the activity of the native protein.

The advantages of the mammary gland as a system for the expression of recombinant proteins: The mammary gland is the organ of choice for the expression of valuable recombinant proteins because milk is easy to collect in large volumes.

Methods for obtaining transgenic animals: The modern understanding of the regulation of gene expression and the discovery of new tools for gene editing can increase the efficiency of creating bioreactors for animals and help to obtain high concentrations of the target protein.

The advantages of using rabbits as bioreactors producing recombinant proteins in milk: The rabbit is a relatively small animal with a short duration of gestation, puberty and optimal size, capable of producing up to 5 liters of milk per year per female, receiving up to 300 grams of the target protein.

Keywords:

genetically modified animals, recombinant protein, bio-pharmacology

Author Contribution

Alexey V. Deykin, Belgorod State National Research University

PhD in Biological Sciences, Director of the Joint Center for Genetic Technologies of Belgorod State National Research University. e-mail: alexei@deikin.ru; ORCID ID http://orcid.org/0000-0001-9960-0863. The author set research objectives, critical analysis of intellectual content, and gave the final approval of the version of the article.

Olesya V. Shcheblykina, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. e-mail: sheolvi31@gmail.com; ORCID ID https://orcid.org/0000-0003-0346-9835. The author was engaged in literature analysis and paper writing.

Elena E. Povetka, Belgorod State National Research University

Medical student, Belgorod State National Research University. e-mail: povetkae@bk.ru, ORCID ID https://orcid.org/0000-0003-4517-1041. The author was engaged in structuring the article and arranging the references.

Polina A. Golubinskaya, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. е-mail: polinapigeon@gmail.com, ORCID ID http://orcid.org/0000-0002-1765-9042. The author collected information and paper writing.

Vladimir M. Pokrovskiy, Belgorod State National Research University

Medical student, Belgorod State National Research University. e-mail: vmpokrovsky@yandex.ru, ORCID ID http://orcid.org/0000-0003-3138-2075. The author made a contribution to collection, analysis and interpretation of the data for the paper.

Liliya V. Korokina, Belgorod State National Research University.

PhD in Medical Sciences, Associate Professor of the Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. ORCID ID http://orcid.org/0000-0002-4115-1564; e-mail: korokina@bsu.edu.ru. The author was engaged in developing the concept and conducting the literature analysis.

Olesya A. Vanchenko, Belgorod State National Research University

Medical student, Belgorod State National Research University. e-mail: 1189889@bsu.edu.ru. The author was engaged in collection, analysis and interpretation of the data for the paper.

Elena V. Kuzubova, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. ORCID ID http://orcid.org/0000-0003-2425-5027; e-mail: 1015artek1015@mail.ru. The author was engaged in collecting the material for the article.

Konstantin S. Trunov, Belgorod State National Research University

Medical student, Belgorod State National Research University. e-mail: 190540789@bsu.edu.ru. The author analyzing the literature.

Viktor V. Vasyutkin, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. e-mail: vivasyutkin@yandex.ru. The author was engaged in collecting the material for the article.

Alexandra I. Radchenko, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. ORCID ID http://orcid.org/0000-0002-4554-2116; e-mail: sandrinkaradchenko@gmail.com. The author analyzing the literature.

Anton P. Danilenko, Belgorod State National Research University

Medical student, Belgorod State National Research University. e-mail: 190540@bsu.edu.ru. The author analyzing the literature.

Julia V. Stepenko, Belgorod State National Research University

postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. ORCID ID http://orcid.org/0000-0002-7414-7326; e-mail: aspirj16@gmail.com. The author analyzing the literature.

Indira S. Kochkarova, Belgorod State National Research University

Postgraduate student, Research Institute of Pharmacology of Living Systems, Belgorod State National Research University. e-mail: kochkarova@bsu.edu.ru. The author was engaged in collection, analysis and interpretation of the data for the paper

Veronika S. Belyaeva, Belgorod State National Research University

Postgraduate student, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. ORCID ID https://orcid.org/0000-0003-2941-0241; e-mail: nika.beliaeva@yandex.ru. The author was engaged in collection, analysis and interpretation of the data for the paper.

Vladimir I. Yakushev, Belgorod State National Research University

PhD in Medicine, Associate Professor, Department of Pharmacology and Clinical Pharmacology, Belgorod State National Research University. e-mail: vladiyakush@yandex.ru. The author analyzing the literature.