Effect of pharmacological preconditioning with incretinomimetics exenatide and vildagliptin on the survival of ischemic tissues

Abstract

Introduction: Currently, much attention is paid to the pleiotropic effects of entretenimiento.

Purposes: Study of the protective effect exenatide and valdiation with pharmacological correction of ischemic myocardial damage, damage of liver and skin graft during the experiment.

Methods: During the experimental study we used a comprehensive approach to the study of the antiischemic effects of entretenimento: doksorubitsinola model of cardiomyopathy, hypo/reperfusion of the isolated heart, ischemia/reperfusion of the liver and the modeling of the skin flap on the supply leg.

Results and discussion: Exenatide (10 mcg/kg/day) and vildagliptin (0.2 mg/kg/day) demonstrate a cardioprotective effect on doxorubicinol model of pathology that is reflected in the decline in the rate of diastolic dysfunction (StТТI), respectively, to 5.3±0.1 standard units. and 6.5±0.2 standart units in comparison with the control group 8.3±0.1 standart units in the model hypo/reperfusion of the isolated hearts of rats, exenatide (10-6 mol/l) and vildagliptin (10-4 mol/l), prevent the decrease of left ventricular pressure (LG). Exenatide (10 μg/kg) and vildagliptin (0.2 mg/kg) prevent necrotization of the skin flap 1.5 and 1.3 times in comparison with the control group. In the model of ischemia/reperfusion of the liver exenatide possess dose related hepatoprotective effect. All protective effects of entretenimiento leveled combined with a blocker of ATP-sensitive potassium channels glibenclamide (0.4 mg/kg).

Conclusion: During the study it was found that exenatide dose of 10 μg/kg/day and vildagliptin dose of 0.2 mg/kg, have a pronounced cardioprotective, hepatoprotective, and a pronounced cytoprotective effect on a model of isolated skin flap on the supply leg. ATP-dependent potassium channels are effector mechanism in the implementation of the protective effects of entretenimiento.