Validation of a mouse model with Apoe gene knockout

Abstract

Introduction: The research focuses on the ApoE-/- transgenic mouse model. This model enhances the understanding of the mechanisms underlying disease progression and allows for the testing of new therapeutic strategies.

Materials and Methods: The study employed ApoE (-/-) knockout mice, which were bred to create cohorts of male mice. These males were divided into two equal groups, receiving either a standard diet or a high-fat diet. Analyses included lipid profile assessments, fasting glucose levels, and evaluation of aortic lesion area.

Results and Discussion: The findings revealed statistically significant differences in the ages at which disease symptoms appeared between the groups. The high-fat diet induced hyperlipidemia, leading to accelerated atherosclerosis in the aorta, aligning with existing literature.

Conclusion: ApoE knockout mice represent a promising genetic model for assessing therapeutic approaches to treat cardiovascular diseases. This model exhibits clear phenotypic manifestations of the disease and can be used for pharmacological correction of endothelial dysfunction.

Graphical Abstract