Cytotoxicity study of transdermal desloratadine delivery system on murine melanoma and human dermal fibroblast cell cultures

Екатерина П. Бродовская; Амина М. Аль-Хадж Аюб; Дмитрий О. Семиков; Валентин П. Агеев; Андрей В. Заборовский; Лариса A. Тарарина; Дина В. Юнина; Сергей А. Литвинов; Изабелла М. Дадаева; Наталия В. Хеладзе; Дмитрий Н. Андреев; Елена Г. Лобанова; Николаев А. Пятаев

doi:10.18413/rrpharmacology.11.731

Авторы

Екатерина П. Бродовская Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва https://orcid.org/0000-0002-1060-9843
Амина М. Аль-Хадж Аюб Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва https://orcid.org/0000-0002-2793-6760
Дмитрий О. Семиков Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва https://orcid.org/0009-0000-7001-6954
Валентин П. Агеев Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва https://orcid.org/0000-0001-5152-5358
Андрей В. Заборовский Российский медицинский университет Минздрава России https://orcid.org/0000-0002-7923-9916
Лариса A. Тарарина Российский медицинский университет Минздрава России https://orcid.org/0000-0001-8006-698X
Дина В. Юнина https://orcid.org/0000-0001-8901-9557
Сергей А. Литвинов Российский медицинский университет Минздрава России https://orcid.org/0009-0007-0716-9924
Изабелла М. Дадаева Российский медицинский университет Минздрава России https://orcid.org/0009-0006-9322-8769
Наталия В. Хеладзе Российский медицинский университет Минздрава России https://orcid.org/0009-0007-9260-5582
Дмитрий Н. Андреев Российский медицинский университет Минздрава России https://orcid.org/0000-0002-4007-7112
Елена Г. Лобанова Российский медицинский университет Минздрава России https://orcid.org/0000-0002-3426-1853
Николаев А. Пятаев Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва https://orcid.org/0000-0002-9688-7640

DOI:

https://doi.org/10.18413/rrpharmacology.11.731

Аннотация

treat and prevent allergic conditions, reducing the adverse effects associated with oral administration.

Materials and Methods: We evaluated the cytotoxicity of the transdermal desloratadinedelivery system in micellar and ionic forms on murine melanoma and human dermal fibroblast cell cultures using the MTT assay and resazurin live cell assay.

Results: The results of the MTT assay demonstrated that desloratadine in micellar and ionic forms had a more pronounced cytotoxic effect on murine melanoma cells than on human dermal fibroblasts. The transdermal system had no effect on cell viability. Desloratadine in micellar or ionic form reduced cell viability: the survival rate of murine melanoma cells was below 50%, while when incubated with the transdermal system, the viability of human dermal fibroblasts was above 70%, indicating no toxicity.

Discussion: The polyvinylpyrrolidone-based transdermal system is not cytotoxic, but the active ingredient, desloratadine, features antiproliferative activity, to a greater extent, in relation to tumor cells.

Conclusion: The obtained results demonstrated the cytotoxic effect of micellar and ionic desloratadine on the tumor culture of murine melanoma and the biocompatibility of the transdermal desloratadine delivery system with human dermal fibroblasts.

Графическая аннотация

Ключевые слова:

antihistamine, biocompatibility, human dermal fibroblasts, murine melanoma, patch, cytotoxicity

Библиографические ссылки

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Вклад авторов

Екатерина П. Бродовская, Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва

Candidate of Medical Sciences, Associate Professor of the Department of Anesthesiology and Resuscitation with courses in Valeology, Life Safety and Disaster Medicine of the Medical Institute, National Research Ogarev Mordovia State University, 68 Bolshevistskaya St., Saransk 430005 Russia; e-mail: kitten-777@mail.ru; ORCID ID: https://orcid.org/0000-0002-1060-9843. Drawing up a research plan and general guidance, designing an experiment, conducting an experiment, writing the text of an article, and conducting an analysis of literary data.

Амина М. Аль-Хадж Аюб, Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва

Engineer of Pharmacokinetics and Targeted Pharmacotherapy Laboratories, National Research Ogarev Mordovia State University, 68 Bolshevistskaya St., Saransk 430005 Russia; e-mail: amina.aioub1@gmail.com; ORCID ID: https://orcid.org/0000-0002-2793-6760. Conducting an experiment, analysis of clinical manifestations, and statistical analysis of data.

Дмитрий О. Семиков, Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва

Research assistant of Laboratory of Preclinical and Clinical Trials of Targeted Forms of Pharmaceuticals, National Research Ogarev Mordovia State University, 68 Bolshevistskaya St., Saransk 430005 Russia; e-mail: dimaj.semikov@yandex.ru; ORCID ID: https://orcid.org/0009-0000-7001-6954. Conducting an experiment, analysis of clinical manifestations, and statistical analysis of data.

Валентин П. Агеев, Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва

Head of Laboratory of Preclinical and Clinical Trials of Targeted Forms of Pharmaceuticals, National Research Ogarev Mordovia State University, 68 Bolshevistskaya Str, Saransk 430005 Russia; e-mail: valeageev@yandex.ru; ORCID ID: https://orcid.org/0000-0001-5152-5358. Conducting an experiment, manufacturing a transdermal therapeutic system for research, and writing the text of an article.

Андрей В. Заборовский, Российский медицинский университет Минздрава России

Doctor Habil. of Medicine, Associate Professor, Head of the Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 4127006 Russia; e-mail: azabor@mail.ru; ORCID ID:https://orcid.org/0000-0002-7923-9916. Developing the experiment design, writing the text of the article, and conducting an analysis of literary data.

Лариса A. Тарарина, Российский медицинский университет Минздрава России

Senior Lecturer of Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 4127006 Russia; e-mail: 79104906528@yandex.ru; ORCID ID: https://orcid.org/0000-0001-8006-698X. Developing the experiment design, conducting an experiment, writing the text of an article, and conducting an analysis of literary data.

Дина В. Юнина

Lecturer of Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 4127006 Russia; e-mail: yunina@msmsu.ru; ORCID ID: https://orcid.org/0000-0001-8901-9557. Developing experiment design, writing the text of the article, and conducting an analysis of literary data.

Сергей А. Литвинов, Российский медицинский университет Минздрава России

PhD in Medicine, Associate Professor of Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 127006 Russia; e-mail: dagar52@mail.ru; ORCID ID: https://orcid.org/0009-0007-0716-9924. Writing the text of the article and conducting an analysis of literary data.

Изабелла М. Дадаева, Российский медицинский университет Минздрава России

Lecturer of Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 1270064 Russia; e-mail: shbellakh@mail.ru; ORCID ID: https://orcid.org/0009-0006-9322-8769. Conducting an analysis of literary data.

Наталия В. Хеладзе, Российский медицинский университет Минздрава России

Lecturer of Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 4127006 Russia; e-mail: 7.053@mail.ru; ORCID ID: https://orcid.org/0009-0007-9260-5582. Conducting an analysis of literary data.

Дмитрий Н. Андреев, Российский медицинский университет Минздрава России

PhD in Medicine, Associate Pprofessor of Department of Propaedeutics of Internal Diseases and Gastroenterology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 4127006 Russia; e-mail: dna-mit8@mail.ru; ORCID ID: https://orcid.org/0000-0002-4007-7112. Conducting an analysis of literary data.

Елена Г. Лобанова, Российский медицинский университет Минздрава России

Doctor Habil. of Medicine, Professor, Department of Pharmacology, Russian University of Medicine of The Ministry of Health of the Russian Federation, 4 Dolgorukovskaya St., Moscow 127006 Russia; e-mail: e.g.lobanova@mail.ru; ORCID ID: https://orcid.org/0000-0002-3426-1853. Conducting an analysis of clinical manifestations and statistical analysis of data.

Николаев А. Пятаев, Национальный исследовательский Мордовский государственный университет имени Н.П. Огарёва

Doctor Habil. of Medicine., Associate Professor, Head of the Department of Anesthesiology and Resuscitation with courses in Valeology, Life Safety and Disaster Medicine, National Research Ogarev Mordovia State University, 68 Bolshevistskaya St., Saransk 430005 Russia; e-mail: pyataevna@mail.ru; ORCID ID: https://orcid.org/0000-0002-9688-7640. Drawing up a research plan and general guidance, developing the design of the experiment, writing the text of the article and analyzing literary data.