Comparative assessment of the effects of Soderm®-Forte, Cytoflavin® and their combination on microcirculation in the gingival mucosa in rats

Authors

DOI:

https://doi.org/10.18413/rrpharmacology.9.10038

Abstract

Introduction: Changes in the microcirculation (MC) system reflect abnormalities of physiological processes in the body and serve as a diagnostic and prognostic factor in a number of pathological conditions, including inflammatory lesions of periodontal tissues. Since in the pathogenesis of microcirculatory disorders an important role is played by the processes of free-radical oxidation, medications with antioxidant effects are of certain interest in terms of pharmacological correction of blood flow disorders in the periodontal complex. It was of interest to conduct a comparative assessment of the effects of Soderm®-Forte, Cytoflavin® and their combination on MC in the gingival mucosa in rats. The aim of the study was to identify whether Soderm®-Forte, Cytoflavin® and their combination can improve microcirculation in the gingival mucosa in rats.

Materials and Methods: The study of MC in the gingival mucosa in rats was carried out by using the laser Doppler Flowmetry method. Four groups of rats with 10 animals in each were formed: Group 1 – control; Groups 2-4 – experimental groups, in which Soderm®-Forte gel was applied to the gingival mucosa of the lower incisors, Cytoflavin®(100 mg/kg intraperitoneally – i.p.) was injected, and gel Soderm®-Forte was applied to the gingival mucosa against the background of injecting Cytoflavin®, respectively. The indicators of blood flow were recorded 30 and 60 minutes later.

Results and Discussion: Soderm®-Fote improved MC in the gingival mucosa by increasing the basal level of NO, the activity of precapillary sphincters and metarteriolas and decreasing the activity of adrenergic vasomotors. Cytoflavin®increased the activity of precapillary sphincters and metarteriolas, the extravascular components of MC, without changing perfusion of the gingival mucosa. The combination of Soderm®-Forte and Cytoflavin® improved MC to a lesser extent than only Soderm®-Forte, but to a bigger extend than Cytoflavin®.

Conclusion: According to the capacity to increase blood flow in the gingival mucosa in rats, the tested substances and their combination can be arranged in the following way: Soderm®-Forte > Soderm®-Forte+Cytoflavin® > Cytoflavin®.

Graphical Abstract

Graphical Abstratc

Keywords:

Laser-Doppler flowmetry, microcirculation, Soderm®-Forte, Cytoflavin®

References

Afanasyev VV, Barantsevich ER, Vishnevetskaya TP (2016) Azbuka neyrotsitoprotektsii [The Essentials of Neurocytoprotection]. Strela, St. Petersburg, 279 pp. [in Russia]

Bains R, Bains V (2015) The antioxidant master glutathione and periodontal health. Dental Research Journal 12(5): 389-405. https://doi.org/10.4103/1735-3327.166169 [PubMed] [PMC]

Borstahl GEO, Oberley-Deegan RE (2018) Superoxide dismutases (SODs) and SOD mimetics. Antioxidants (Basel) 7(11): 156. https://doi.org/10.3390/antiox7110156 [PubMed] [PMC]

Chutko LS, Surushkina SYu, Yakovenko EA, Anisimova TI, Prokopenko SM (2017) Issledovaniye effektivnosti tsitoflavina v lechenii somatoformnykh rasstroystv [Study of the effectiveness of cytoflavin when treating somatoform disorders]. S.S. Korsakov Journal of Neurology and Psychiatry [Zhurnal Nevrologii i Psikhiatrii im. S.S. Korsakova] 1: 21–24. https://doi.org/10.17116/jnevro20171171121-24

Chutko LS, Surushkina SYu, Yakovenko EA, Rozhkova AV, Volov MB, Zhukovskaya NV, Madzhidova EN (2019) Vozmozhnosti primeneniya Tsitoflavina pri lechenii kognitivnykh i emotsional’nykh narusheniy u patsiyentov s golovnymi bolyami napryazheniya [Possibilities of using Cytoflavin when treating cognitive and emotional disorders in patients with tension headaches]. Zhurnal nevrologii i psikhiatrii im. S.S. Korsakova [S.S. Korsakov Journal of Neurology and Psychiatry] 119 (11): 32-36. https://doi.org/10.17116/jnevro201911911132

Coudriet GM, Delmastro-Greenwood MM, Previte DM, Marré ML, O’Connor EC, Novak EA, Vincent G, Mollen KP, Lee S, Dong H, Piganelli JD (2017) Treatment with a catalytic superoxide dismutase (SOD) mimetic improves liver steatosis, insulin sensitivity, and inflammation in obesity-induced type 2 diabetes. Antioxidants 6(4): 85. https://doi.org/10.3390/antiox6040085 [PubMed] [PMC]

Galenko-Yaroshevskiy PA, Tseluyko KV, Pavlyuchenko II, Leont’iev VK, Zadorozhniy AV, Popkov VL, Lebedeva SA, Zelenskaya AV, Zadorozhniy MA, Zobenko VYa (2022a) Vliyaniye traditsionnoy medikamentoznoy terapii i sochetaniya yeyo s Soderm®-Forte i novoy in’yektsionnoy formoy Reksoda® na sostoyaniye antioksidantno-prooksidantnoy sistemy eritrotsitov krovi pri eksperimental’nom parodontite u krys [The impact of traditional drug therapy and its combination with Soderm®-Forte and a new Rexod® injectable form on the state of the antioxidant-prooxidant system of red blood cells in experimental periodontitis in rats]. Kuban Scientific Medical Bulletin [Kubanskiy Nauchnyy Meditsinskiy Vestnik] 29(4): 32–52. https://doi.org/10.25207/1608-6228-2022-29-4-32-52 [in Russian]

Galenko-Yaroshevsky PA, Leontev VK, Slavinskiy AА, Tseluiko KV, Zadorozhniy MA, Todorov SS, Popkov VL, Lebedeva SА, Zelenskaya AV, Zadorozhniy AV, Verevkin AА (2022b) The effect of the composition of Soderm®-Forte gel and the new injectable form of Rexod® on pathology findings in gingival tissue in experimental periodontitis in rats. Research Results in Pharmacology 8(4): 141–156. https://doi.org/10.3897/rrpharmacology.8.98809

Iskra DA (2016) Patogheneticheskiye aspekty primeneniya tsitoflavina v lechenii khronicheskoy posttravmaticheskoy golovnoy boli [Pathogenetic aspects of using Cytoflavin in the treatment of chronic post-traumatic headache]. S.S. Korsakov Journal of Neurology and Psychiatry [Zhurnal Nevrologii i Psikhiatrii im. S.S. Korsakova] 116(11): 41–44. https://doi.org/10.17116/jnevro201611611141-44 [in Russian]

Kamchatnov PR, Chugunov AV, Osmayeva ZKh (2019) Tsitoflavin: vozmozhnosti metabolicheskoy terapii u bol’nykh s distsirkulyatornoy entsefalopatiyey [Cytoflavin: possibilities of metabolic therapy in patients with dyscirculatory encephalopathy]. Doctor.Ru 1(156): 14–19. https://doi.org/10.31550/1727-2378-2019-156-1-14-19 [in Russian]

Krupatkin AI (2018) Znacheniya kolebatel’nykh protsessov v diagnostike sostoyaniya mikrotsirkulyatorno-tkanevykh system [The role of oscillatory processes in the diagnosis of the state of microcirculatory tissue systems]. Fiziologiya Cheloveka [Physiology of Man] 44(5): 103–114. https://doi.org/10.1134/S0131164618050077 [in Russian]

Krupatkin AI, Dorokhin AI, Adrianova AA (2022) Rol’ mikrotsirkulyatsii kostnoy tkani v yeye zhiznedeyatel’nosti i reparativnoy regheneratsii [The role of microcirculation of bone tissue in its activity and reparative regeneration]. Sovremennyye Metody Neinvazivnogo Issledovaniya. Regionarnoye Krovoobrashcheniye i Mikrotsirkulyatsiya [Modern Methods of Non-invasive research. Regional Circulation and Microcirculation] 21(1): 12–17. https://doi.org/10.24884/1682-6655-2022-21-1-12-17 [in Russian]

Krupatkin AI, Sidorov VV (2005) Lazernaya Dopplerovskaya Floumetriya Mikrotsirkulyatsii Krovi [Laser Doppler Flowmetry of Blood Microcirculation]. Meditsina, Moskva, 254 pp. [in Russian]

Krupatkin AI, Sidorov VV (2013) Funktsional’naya Diagnostika Sostoyaniya Mikrotsirkulyatorno-tkanevykh Sistem: Kolebaniya, Informatsiya, Nelineynost’ [Functional Diagnostics of the State of Microcirculatory Tissue Systems: Oscillations, Information, and Non-linearity]. Guide for doctors. Librokom, Moscow, 496 pp. [in Russian]

Kwiecien S, Jasnos K, Magierowski M, Sliwowski Z, Pajdo R, Brzozowski B, Mach T, Wojcik D, Brzozowski T (2014) Lipid peroxidation, reactive oxygen species and antioxidative factors in the pathogenesis of gastric mucosal lesions and mechanism of protection against oxidative stress - induced gastric injury. Journal of Physiology and Pharmacology 65(5): 613–622. [PubMed]

Lengert EV, Savkina AA, Ermakov AV, Saveleva MS, Lagutina DD, Stepanova TV, Ivanov AN (2021) Influence of the new formulation based on silver alginate microcapsules loaded with tannic acid on the microcirculation of the experimental periodontitis in rats. Materials Science and Engineering 126: 112144. https://doi.org/10.1016/j.msec.2021.112144 [PubMed]

Lira-Junior R, Figueredo CM, Bouskela E, Fischer RG (2014) Severe chronic periodontitis is associated with endothelial and microvascular dysfunctions: a pilot study. Journal of Periodontology 85 (12): 1648–1657. https://doi.org/10.1902/jop.2014.140189 [PubMed]

Mansuroglu B, Dermanb S, Yabac A, Kızılbey K (2015) Protective effect of chemically modified SOD on lipid peroxidation and antioxidant status in diabetic rats. International Journal of Biological Macromolecules 72: 79–87. https://doi.org/10.1016/j.ijbiomac.2014.07.039 [PubMed]

Policar C, Bouvet J, Bertrand HC, Delsuc N (2022) SOD mimics: From the tool box of the chemists to cellular studies. Current Opinion in Chemical Biology 67: 102109. https://doi.org/10.1016/j.cbpa.2021.102109 [PubMed]

Popkov VL, Zadorozhnyy MA, Kheygetyan AV, Galenko-Yaroshevskiy PA (2022) Vliyaniye kompozitsii gelya Soderm®-Forte i metabolicheskogo energokorrektora tsitoflavina na techeniye vospalitel’nogo protsessa v usloviyakh eksperimental’nogo parodontita [The influence of the composition of Soderm®-Forte gel and metabolic energy corrector Cytoflavin on the course of the inflammatory process in conditions of experimental periodontitis]. Stomatologiya dlya Vsekh [Dentistry for All] 2(99): 46–53. https://doi.org/10.35556/idr-2022-2(99)46-53 [in Russian]

Ravayeva MYu, Chuyan EN, Biryukova EA, Albaeva RN, Faychak SN (2018) Pokazateli mikrotsirkulyatsii krys, nakhodyashchikhsya v usloviyakh kombinirovannogo deystviya khronicheskogo i ostrogo stressa [Indicators of microcirculation in rats in the conditions of combined chronic and acute stress]. Orbital’ 2(3): 1–7. [in Russian]

Shakhmardanova SA, Gulevskaya ON, Seletskaya VV, Zelenskaya AV, Khananashvili YaA, Nefedov DA, Galenko-Yaroshevskiy PA (2016) Antioksidanty: klassifikatsiya, farmakoterapevticheskiye svoystva, ispol’zovaniye v prakticheskoy meditsinе [Antioxidants: classification, pharmacotherapeutic properties, use in practical medicine]. Zhurnal Fundamental’noy Meditsiny i Biologii [Journal of Fundamental Medicine and Biology]. 3: 4–15.

Tóthová L, Celec P (2017) Oxidative stress and antioxidants in the diagnosis and therapy of periodontitis. Frontiers in Physiology 8. https://doi.org/10.3389/fphys.2017.01055 [PubMed] [PMC]

Tsubokawa M, Sato S (2014) In vitro analysis of human periodontal microvascular endothelial cells. Journal of Periodontology 85: 1135–1142. https://doi.org/10.1902/jop.2013.130209 [PubMed]

Zyul’kina LA, Sabayeva MN, Ivanov PV, Shastin YEN (2017) Mikrotsirkulyatsiya tkaney parodonta: prichiny narusheniy i mekhanizmy korrektsii [Microcirculation of periodontal tissues: causes of disorders and correction mechanisms]. Modern Problems of Science and Education [Sovremennyye Problemy Nauki i Obrazovaniya] 2. URL:https://science-education.ru/ru/article/view?id=26259 [in Russian]

Author Contribution

Pavel A. Galenko-Yaroshevsky, Kuban State Medical University

Сorresponding member of the Russian Academy of Sciences, Holder of an Advanced Doctorate (Doctor of Science) in Medical Sciences, Professor, Head of the Department of Pharmacology of Kuban State Medical University, Krasnodar, Russia; Senior Research Fellow of Research Institute for the Pharmacology of Living Systems, Belgorod State National Research University, Belgorod, Russia; е-mail: Galenko.Yaroсhevsky@gmail.com; ORCID ID https://orсid.org/0000-0001-6856-1777. The author’s contribution: concept development, formulation and development of the key aim and objectives; interpretations of the data obtained; critical review of the draft copy and provision of valuable comments; participation in preparing the final version of the paper in terms of visualization and data presentation; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Marina Yu. Ravaeva, V.I. Vernadsky Crimean Federal University

PhD in Biological Sciences, Associate Professor, Department of Physiology of Humans and Animals and Biophysics, Faculty of Biology and Chemistry, V.I. Vernadsky Crimean Federal University, Simferopol, Russia; e-mail: ravaevam@yandex.ru; ORCID ID https://orcid.org/0000-0002-6081-1628. The author’s contribution: concept development, formulation and development of the key aim and objectives; interpretation of the data obtained; critical review of the draft copy and provision of valuable comments; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Elena N. Chuyan, V.I. Vernadsky Crimean Federal University

Professor, Head of the Department of Physiology of Humans and Animals and Biophysics, Faculty of Biology and Chemistry, V.I. Vernadsky Crimean Federal University, Simferopol, Russia; e-mail: Elena-chuyan@rambler.ru; ORCID ID https://orcid.org/0000-0001-6240-2732. The author’s contribution: concept development, formulation and development of the key aim and objectives; interpretation of the data obtained; writing a draft manuscript, its critical review and providing valuable comments; participation in research design; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Mark A. Zadorozhniy, Rostov State Medical University

Postgraduate student, Department of Dentistry No.1, Rostov State Medical University, Rostov-on-Don, Russia; e-mail: markys-97@yandex.ru; ORCID ID https://orcid.org/0000-0002-7528-6109. The author’s contribution: concept development, formulation and development of the key aim and objectives; analysis and interpretation of the data obtained; critical review of the draft copy and provision of valuable comments; participation in research design; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Victor L. Popkov, Kuban State Medical University

Holder of an Advanced Doctorate (Doctor of Science) in Medical Sciences, Professor of the Department of Orthopedic Dentistry, Kuban State Medical University, Krasnodar, Russia; e-mail: Vict.popkoff2015@yandex.ru; ORCID ID https://orсid.org/0000-0001-7362-0073. The author’s contribution: concept development, formulation and development of the key aim and objectives; interpretation of the data obtained; writing a draft manuscript, its critical review and providing valuable comments; participation in research design; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Anait V. Zelenskaya, Kuban State Medical University

PhD in Medical Sciences, Associate professor, Department of Pharmacology, Kuban State Medical University, Krasnodar, Russia; e-mail: Anait_06@mail.ru; ORCID ID https://orсid.org/0000-0001-9512-2526. The author’s contribution: concept development, formulation and development of the key aim and objectives; interpretation of the data obtained; critical review of the draft manuscript and providing valuable comments; participation in research design; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper.

Artur V. Kheigetyan, Rostov State Medical University

PhD in Medical Sciences, Associate Professor, Head of the Department of Dentistry No.1, Rostov State Medical University, Rostov-on-Don, Russia; e-mail: artur5953@yandex.ru; ORCID ID https://orcid.org/0000-0002-8222-4854. The author’s contribution: development of the key aim and objectives; search and analysis of literature on the topic of research; writing a draft manuscript and forming its final version; participation in research design.

Lusine O. Alukhanyan, Kuban State Medical University

PhD in Medical Sciences, Associate Professor, Department of Pediatric Dentistry, Orthodontics and Maxillofacial Surgery, Kuban State Medical University, Krasnodar, Russia; e-mail: LUS.0912@mail.ru; ORCID ID https://orсid.org/0009-0005-1752-2491.  The author’s contribution: conducting research; analysis and interpretation of the data obtained; participation in research design.

Elizaveta V. Statsenko, Kuban State Medical University

4th-year undergraduate student, Faculty of Dentistry, Kuban State Medical University, Krasnodar, Russia; e-mail: liza.statsenko.0202@mail.ru;  ORCID ID https://orсid.org/0009-0009-3020-7401. The author’s contribution: development of the key aim and objectives; writing a draft manuscript; participation in research design; participation in preparing the final version of the paper in terms of visualization and data presentation.

Maria Yu. Materenchuk, Sechenov University

5th-year undergraduate student, Institute of Biodesign and Complex System Simulation, Sechenov University, Moscow, Russia; e-mail: mariamatter231@gmail.com; ORCID ID https://orcid.org/0000-0002-0711-4153. The author’s contribution: development of the key aim and objectives; writing a draft manuscript; participation in research design; participation in preparing the final version of the paper in terms of visualization and data presentation.

Svetlana A. Lebedeva, Sechenov University

Holder of an Advanced Doctorate (Doctor of Science) in  Biological Sciences, Associate Professor, Professor of the Department of Pharmacology, A.P. Nelyubin Institute of Pharmacy, Sechenov University, Moscow, Russia; e-mail: lebedeva502@yandex.ru; ORCID ID https://orcid.org/0000-0003-0325-6397. The author’s contribution: development of the key aim and objectives; writing a draft manuscript; participation in research design; being in charge of all aspects of the study, cohesion of its parts and the final version of the paper; applying statistical methods for analysis and synthesis of the data.

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Published

19-07-2023

How to Cite

Galenko-Yaroshevsky PA, Ravaeva MY, Chuyan EN, Zadorozhniy MA, Popkov VL, Zelenskaya AV, Kheigetyan AV, Alukhanyan LO, Statsenko EV, Materenchuk MY, Lebedeva SA (2023) Comparative assessment of the effects of Soderm®-Forte, Cytoflavin® and their combination on microcirculation in the gingival mucosa in rats. Research Results in Pharmacology 9(3): 11–20. https://doi.org/10.18413/rrpharmacology.9.10038

Issue

Vol. 9 No. 3 (2023)

Section

Experimental Pharmacology

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